Release probability of hippocampal glutamatergic terminals scales with the size of the active zone

Cortical synapses display remarkable structural, molecular and functional heterogeneity. Our knowledge regarding the relationship between the ultrastructural and functional parameters is still fragmented. Here we asked how the release probability and presynaptic [Ca2+] transients relate to the ultra...

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Main Authors:	Holderith, Noemi, Lorincz, Andrea, Katona, Gergely, Rózsa, Balázs, Kulik, Akos, Watanabe, Masahiko, Nusser, Zoltan
Format:	Online
Language:	English
Published:	2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386897/

id	pubmed-3386897
recordtype	oai_dc
spelling	pubmed-33868972013-01-01 Release probability of hippocampal glutamatergic terminals scales with the size of the active zone Holderith, Noemi Lorincz, Andrea Katona, Gergely Rózsa, Balázs Kulik, Akos Watanabe, Masahiko Nusser, Zoltan Article Cortical synapses display remarkable structural, molecular and functional heterogeneity. Our knowledge regarding the relationship between the ultrastructural and functional parameters is still fragmented. Here we asked how the release probability and presynaptic [Ca2+] transients relate to the ultrastructure of rat hippocampal glutamatergic axon terminals. Two-photon Ca2+ imaging-derived optical quantal analysis and correlated electron microscopic reconstructions revealed a tight correlation between the release probability and the active zone area. The peak amplitude of [Ca2+] transients in single boutons also positively correlated with the active zone area. Freeze-fracture immunogold labeling revealed that the voltage-gated Ca2+ channel subunit Cav2.1 and the presynaptic protein Rim1/2 are confined to the active zone and their numbers scale linearly with the active zone area. Gold particles for Cav2.1 showed a nonrandom distribution within the active zones. Our results demonstrate that the number of several active zone proteins, including presynaptic Ca2+ channels, docked vesicles and the release probability scales linearly with the active zone area. 2012-06-10 /pmc/articles/PMC3386897/ /pubmed/22683683 http://dx.doi.org/10.1038/nn.3137 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Holderith, Noemi Lorincz, Andrea Katona, Gergely Rózsa, Balázs Kulik, Akos Watanabe, Masahiko Nusser, Zoltan
spellingShingle	Holderith, Noemi Lorincz, Andrea Katona, Gergely Rózsa, Balázs Kulik, Akos Watanabe, Masahiko Nusser, Zoltan Release probability of hippocampal glutamatergic terminals scales with the size of the active zone
author_facet	Holderith, Noemi Lorincz, Andrea Katona, Gergely Rózsa, Balázs Kulik, Akos Watanabe, Masahiko Nusser, Zoltan
author_sort	Holderith, Noemi
title	Release probability of hippocampal glutamatergic terminals scales with the size of the active zone
title_short	Release probability of hippocampal glutamatergic terminals scales with the size of the active zone
title_full	Release probability of hippocampal glutamatergic terminals scales with the size of the active zone
title_fullStr	Release probability of hippocampal glutamatergic terminals scales with the size of the active zone
title_full_unstemmed	Release probability of hippocampal glutamatergic terminals scales with the size of the active zone
title_sort	release probability of hippocampal glutamatergic terminals scales with the size of the active zone
description	Cortical synapses display remarkable structural, molecular and functional heterogeneity. Our knowledge regarding the relationship between the ultrastructural and functional parameters is still fragmented. Here we asked how the release probability and presynaptic [Ca2+] transients relate to the ultrastructure of rat hippocampal glutamatergic axon terminals. Two-photon Ca2+ imaging-derived optical quantal analysis and correlated electron microscopic reconstructions revealed a tight correlation between the release probability and the active zone area. The peak amplitude of [Ca2+] transients in single boutons also positively correlated with the active zone area. Freeze-fracture immunogold labeling revealed that the voltage-gated Ca2+ channel subunit Cav2.1 and the presynaptic protein Rim1/2 are confined to the active zone and their numbers scale linearly with the active zone area. Gold particles for Cav2.1 showed a nonrandom distribution within the active zones. Our results demonstrate that the number of several active zone proteins, including presynaptic Ca2+ channels, docked vesicles and the release probability scales linearly with the active zone area.
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386897/
_version_	1611540242298306560

Release probability of hippocampal glutamatergic terminals scales with the size of the active zone

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