Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea
Genetic diversity and antiviral activity for five common antiviral drugs of echovirus (ECV) 5 isolated in Korea have been described. The present study extended these tests to a Korean ECV 18 isolate. An outbreak of aseptic meningitis caused by the ECV 18 isolate was reported in Korea in 2005, markin...
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BioMed Central
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377930/ |
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pubmed-33779302012-06-20 Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea Park, KwiSung Yeo, SangGu Baek, KyoungAh Cheon, DooSung Choi, YoungJin Park, JoonSoo Lee, SooJin Short Report Genetic diversity and antiviral activity for five common antiviral drugs of echovirus (ECV) 5 isolated in Korea have been described. The present study extended these tests to a Korean ECV 18 isolate. An outbreak of aseptic meningitis caused by the ECV 18 isolate was reported in Korea in 2005, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from stool specimen of a 5-year-old male patient with aseptic meningitis, the complete genome sequence was obtained and was compared it with the Metcalf prototype strain. Unlike the ECV5 isolate, the 3' untranslated region had the highest identity value (94.2%) at the nucleotide level, while, at the amino acid level, the P2 region displayed the highest identity value (96.9%). These two strains shared all cleavage sites, with the exception of the 2B/2C site, which was RQ/NN in the Metcalf strain but RQ/NS in the Korean ECV 18 isolate. In Vero cells infected with the Korean ECV 18 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC50 value >100 μg/mL. Of the five drugs, only amantadine (IC50: 4.97 ± 0.77 μg/mL, TI: 20.12) and ribavirin (IC50: 7.63 ± 0.87 μg/mL, TI: 13.11) had any antiviral activity against the Korean ECV 18 isolate in the five antiviral drugs. These antiviral activity effects were similar with results of the Korean ECV5 isolate. BioMed Central 2011-11-11 /pmc/articles/PMC3377930/ /pubmed/22078806 http://dx.doi.org/10.1186/1743-422X-8-516 Text en Copyright ©2011 Park et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Park, KwiSung Yeo, SangGu Baek, KyoungAh Cheon, DooSung Choi, YoungJin Park, JoonSoo Lee, SooJin |
| spellingShingle |
Park, KwiSung Yeo, SangGu Baek, KyoungAh Cheon, DooSung Choi, YoungJin Park, JoonSoo Lee, SooJin Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea |
| author_facet |
Park, KwiSung Yeo, SangGu Baek, KyoungAh Cheon, DooSung Choi, YoungJin Park, JoonSoo Lee, SooJin |
| author_sort |
Park, KwiSung |
| title |
Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea |
| title_short |
Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea |
| title_full |
Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea |
| title_fullStr |
Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea |
| title_full_unstemmed |
Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea |
| title_sort |
molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in korea |
| description |
Genetic diversity and antiviral activity for five common antiviral drugs of echovirus (ECV) 5 isolated in Korea have been described. The present study extended these tests to a Korean ECV 18 isolate. An outbreak of aseptic meningitis caused by the ECV 18 isolate was reported in Korea in 2005, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from stool specimen of a 5-year-old male patient with aseptic meningitis, the complete genome sequence was obtained and was compared it with the Metcalf prototype strain. Unlike the ECV5 isolate, the 3' untranslated region had the highest identity value (94.2%) at the nucleotide level, while, at the amino acid level, the P2 region displayed the highest identity value (96.9%). These two strains shared all cleavage sites, with the exception of the 2B/2C site, which was RQ/NN in the Metcalf strain but RQ/NS in the Korean ECV 18 isolate. In Vero cells infected with the Korean ECV 18 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC50 value >100 μg/mL. Of the five drugs, only amantadine (IC50: 4.97 ± 0.77 μg/mL, TI: 20.12) and ribavirin (IC50: 7.63 ± 0.87 μg/mL, TI: 13.11) had any antiviral activity against the Korean ECV 18 isolate in the five antiviral drugs. These antiviral activity effects were similar with results of the Korean ECV5 isolate. |
| publisher |
BioMed Central |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377930/ |
| _version_ |
1611537620720943104 |