RhoB regulates cell migration through altered focal adhesion dynamics

The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension, although they have active membrane ruffling around the periphery. Deple...

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Main Authors:	Vega, Francisco M., Colomba, Audrey, Reymond, Nicolas, Thomas, Mairian, Ridley, Anne J.
Format:	Online
Language:	English
Published:	The Royal Society 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3376739/

id	pubmed-3376739
recordtype	oai_dc
spelling	pubmed-33767392012-06-21 RhoB regulates cell migration through altered focal adhesion dynamics Vega, Francisco M. Colomba, Audrey Reymond, Nicolas Thomas, Mairian Ridley, Anne J. Research The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension, although they have active membrane ruffling around the periphery. Depletion of the exchange factor GEF-H1 induces a similar phenotype. RhoB-depleted cells migrate faster, but less persistently in a chemotactic gradient, and frequently round up during migration. RhoB-depleted cells have similar numbers of focal adhesions to control cells during spreading and migration, but show more diffuse and patchy contact with the substratum. They have lower levels of surface β1 integrin, and β1 integrin activity is reduced in actin-rich protrusions. We propose that RhoB contributes to directional cell migration by regulating β1 integrin surface levels and activity, thereby stabilizing lamellipodial protrusions. The Royal Society 2012-05 /pmc/articles/PMC3376739/ /pubmed/22724071 http://dx.doi.org/10.1098/rsob.120076 Text en http://creativecommons.org/licenses/by/3.0/ © 2012 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Vega, Francisco M. Colomba, Audrey Reymond, Nicolas Thomas, Mairian Ridley, Anne J.
spellingShingle	Vega, Francisco M. Colomba, Audrey Reymond, Nicolas Thomas, Mairian Ridley, Anne J. RhoB regulates cell migration through altered focal adhesion dynamics
author_facet	Vega, Francisco M. Colomba, Audrey Reymond, Nicolas Thomas, Mairian Ridley, Anne J.
author_sort	Vega, Francisco M.
title	RhoB regulates cell migration through altered focal adhesion dynamics
title_short	RhoB regulates cell migration through altered focal adhesion dynamics
title_full	RhoB regulates cell migration through altered focal adhesion dynamics
title_fullStr	RhoB regulates cell migration through altered focal adhesion dynamics
title_full_unstemmed	RhoB regulates cell migration through altered focal adhesion dynamics
title_sort	rhob regulates cell migration through altered focal adhesion dynamics
description	The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension, although they have active membrane ruffling around the periphery. Depletion of the exchange factor GEF-H1 induces a similar phenotype. RhoB-depleted cells migrate faster, but less persistently in a chemotactic gradient, and frequently round up during migration. RhoB-depleted cells have similar numbers of focal adhesions to control cells during spreading and migration, but show more diffuse and patchy contact with the substratum. They have lower levels of surface β1 integrin, and β1 integrin activity is reduced in actin-rich protrusions. We propose that RhoB contributes to directional cell migration by regulating β1 integrin surface levels and activity, thereby stabilizing lamellipodial protrusions.
publisher	The Royal Society
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3376739/
_version_	1611537250607169536

RhoB regulates cell migration through altered focal adhesion dynamics

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