Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Optical Society of America
2010
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369551/ |