Role of vitamin D-binding protein in isocyanate-induced occupational asthma

The development of a serological marker for early diagnosis of isocyanate-induced occupational asthma (isocyanate-OA) may improve clinical outcome. Our previous proteomic study found that expression of vitamin D-binding protein (VDBP) was upregulated in the patients with isocyanate-OA. In the presen...

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Main Authors:	Kim, Sung-Ho, Choi, Gil-Soon, Nam, Young-Hee, Kim, Joo-Hee, Hur, Gyu-Young, Kim, Seung-Hyun, Park, Sang Myun, Park, Hae-Sim
Format:	Online
Language:	English
Published:	Korean Society for Biochemistry and Molecular Biology 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366325/

id	pubmed-3366325
recordtype	oai_dc
spelling	pubmed-33663252012-06-07 Role of vitamin D-binding protein in isocyanate-induced occupational asthma Kim, Sung-Ho Choi, Gil-Soon Nam, Young-Hee Kim, Joo-Hee Hur, Gyu-Young Kim, Seung-Hyun Park, Sang Myun Park, Hae-Sim Original Article The development of a serological marker for early diagnosis of isocyanate-induced occupational asthma (isocyanate-OA) may improve clinical outcome. Our previous proteomic study found that expression of vitamin D-binding protein (VDBP) was upregulated in the patients with isocyanate-OA. In the present study, we evaluated the clinical relevance of VDBP as a serological marker in screening for isocyanate-OA among exposed workers and its role in the pathogenesis of isocyanate-OA. Three study groups including 61 patients with isocyanate-OA (group I), 180 asymptomatic exposed controls (AECs, group II), 58 unexposed healthy controls (NCs, group III) were enrolled in this study. The baseline serum VDBP level was significantly higher in group I compared with groups II and III. The sensitivity and specificity for predicting the phenotype of isocyanate-OA with VDBP were 69% and 81%, respectively. The group I subjects with high VDBP (≥ 311 µg/ml) had significantly lower PC20 methacholine levels than did subjects with low VDBP. The in vitro studies showed that TDI suppressed the uptake of VDBP into RLE-6TN cells, which was mediated by the downregulation of megalin, an endocytic receptor of the 25-hydroxycholecalciferol-VDBP complex. Furthermore, toluene diisocyanate (TDI) increased VEGF production and secretion from this epithelial cells by suppression of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] production. The findings of this study suggest that the serum VDBP level may be used as a serological marker for the detection of isocyanate-OA among workers exposed to isocyanate. The TDI-induced VEGF production/secretion was reversed by 1,25(OH)2D3 treatment, which may provide a potential therapeutic strategy for patients with isocyanate-OA. Korean Society for Biochemistry and Molecular Biology 2012-05-31 2012-02-08 /pmc/articles/PMC3366325/ /pubmed/22314196 http://dx.doi.org/10.3858/emm.2012.44.5.036 Text en Copyright © 2012 by the Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kim, Sung-Ho Choi, Gil-Soon Nam, Young-Hee Kim, Joo-Hee Hur, Gyu-Young Kim, Seung-Hyun Park, Sang Myun Park, Hae-Sim
spellingShingle	Kim, Sung-Ho Choi, Gil-Soon Nam, Young-Hee Kim, Joo-Hee Hur, Gyu-Young Kim, Seung-Hyun Park, Sang Myun Park, Hae-Sim Role of vitamin D-binding protein in isocyanate-induced occupational asthma
author_facet	Kim, Sung-Ho Choi, Gil-Soon Nam, Young-Hee Kim, Joo-Hee Hur, Gyu-Young Kim, Seung-Hyun Park, Sang Myun Park, Hae-Sim
author_sort	Kim, Sung-Ho
title	Role of vitamin D-binding protein in isocyanate-induced occupational asthma
title_short	Role of vitamin D-binding protein in isocyanate-induced occupational asthma
title_full	Role of vitamin D-binding protein in isocyanate-induced occupational asthma
title_fullStr	Role of vitamin D-binding protein in isocyanate-induced occupational asthma
title_full_unstemmed	Role of vitamin D-binding protein in isocyanate-induced occupational asthma
title_sort	role of vitamin d-binding protein in isocyanate-induced occupational asthma
description	The development of a serological marker for early diagnosis of isocyanate-induced occupational asthma (isocyanate-OA) may improve clinical outcome. Our previous proteomic study found that expression of vitamin D-binding protein (VDBP) was upregulated in the patients with isocyanate-OA. In the present study, we evaluated the clinical relevance of VDBP as a serological marker in screening for isocyanate-OA among exposed workers and its role in the pathogenesis of isocyanate-OA. Three study groups including 61 patients with isocyanate-OA (group I), 180 asymptomatic exposed controls (AECs, group II), 58 unexposed healthy controls (NCs, group III) were enrolled in this study. The baseline serum VDBP level was significantly higher in group I compared with groups II and III. The sensitivity and specificity for predicting the phenotype of isocyanate-OA with VDBP were 69% and 81%, respectively. The group I subjects with high VDBP (≥ 311 µg/ml) had significantly lower PC20 methacholine levels than did subjects with low VDBP. The in vitro studies showed that TDI suppressed the uptake of VDBP into RLE-6TN cells, which was mediated by the downregulation of megalin, an endocytic receptor of the 25-hydroxycholecalciferol-VDBP complex. Furthermore, toluene diisocyanate (TDI) increased VEGF production and secretion from this epithelial cells by suppression of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] production. The findings of this study suggest that the serum VDBP level may be used as a serological marker for the detection of isocyanate-OA among workers exposed to isocyanate. The TDI-induced VEGF production/secretion was reversed by 1,25(OH)2D3 treatment, which may provide a potential therapeutic strategy for patients with isocyanate-OA.
publisher	Korean Society for Biochemistry and Molecular Biology
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366325/
_version_	1611534388474937344

Role of vitamin D-binding protein in isocyanate-induced occupational asthma

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