The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate

The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate

Genotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells. The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamic...

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Main Authors: Widziewicz, Kamila, Kalka, Joanna, Skonieczna, Magdalena, Madej, Paweł
Format: Online
Language:English
Published: The Scientific World Journal 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361255/
id pubmed-3361255
recordtype oai_dc
spelling pubmed-33612552012-06-04 The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate Widziewicz, Kamila Kalka, Joanna Skonieczna, Magdalena Madej, Paweł Research Article Genotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells. The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamics. The leachate samples were collected from Zabrze landfill, situated in the Upper Silesian Industrial District, Poland. Statistically significant differences (Kruskal-Wallice ANOVA rank model) were observed between DNA strand breaks in cells incubated with leachate before and after treatment (P < 0.001). Nonparametric Friedman ANOVA confirmed time-reliable and concentration-reliable cells response to leachate concentration. Examinations of chemical properties showed a marked decrease in leachate parameters after treatment which correlate to reduced genotoxicity towards tested cells. Obtained results demonstrate that biological cotreatment of leachate together with municipal wastewater is an efficient method for its genotoxic potential reduction; however, treated leachate still possessed genotoxic character. The Scientific World Journal 2012-04-30 /pmc/articles/PMC3361255/ /pubmed/22666120 http://dx.doi.org/10.1100/2012/435239 Text en Copyright © 2012 Kamila Widziewicz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Widziewicz, Kamila
Kalka, Joanna
Skonieczna, Magdalena
Madej, Paweł
spellingShingle Widziewicz, Kamila
Kalka, Joanna
Skonieczna, Magdalena
Madej, Paweł
The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
author_facet Widziewicz, Kamila
Kalka, Joanna
Skonieczna, Magdalena
Madej, Paweł
author_sort Widziewicz, Kamila
title The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
title_short The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
title_full The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
title_fullStr The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
title_full_unstemmed The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
title_sort comet assay for the evaluation of genotoxic potential of landfill leachate
description Genotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells. The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamics. The leachate samples were collected from Zabrze landfill, situated in the Upper Silesian Industrial District, Poland. Statistically significant differences (Kruskal-Wallice ANOVA rank model) were observed between DNA strand breaks in cells incubated with leachate before and after treatment (P < 0.001). Nonparametric Friedman ANOVA confirmed time-reliable and concentration-reliable cells response to leachate concentration. Examinations of chemical properties showed a marked decrease in leachate parameters after treatment which correlate to reduced genotoxicity towards tested cells. Obtained results demonstrate that biological cotreatment of leachate together with municipal wastewater is an efficient method for its genotoxic potential reduction; however, treated leachate still possessed genotoxic character.
publisher The Scientific World Journal
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361255/
_version_ 1611532765906337792

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