VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase

Tryptases are predominantly mast cell-specific serine proteases with pleiotropic biological activities and play a critical role in skin allergic reactions, which are manifested with rapid edema and increases of vascular permeability. The exact mechanisms of mast cell tryptase promoting vascular perm...

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Main Authors:	Bai, Qianming, Li, Xiaobo, Wang, Xinhong, Xu, Yali, Wang, Li, Zhang, Qingyong, Yin, Lianhua
Format:	Online
Language:	English
Published:	International Scholarly Research Network 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361195/

id	pubmed-3361195
recordtype	oai_dc
spelling	pubmed-33611952012-06-04 VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase Bai, Qianming Li, Xiaobo Wang, Xinhong Xu, Yali Wang, Li Zhang, Qingyong Yin, Lianhua Research Article Tryptases are predominantly mast cell-specific serine proteases with pleiotropic biological activities and play a critical role in skin allergic reactions, which are manifested with rapid edema and increases of vascular permeability. The exact mechanisms of mast cell tryptase promoting vascular permeability, however, are unclear and, therefore, we investigated the effect and mechanism of tryptase or human mast cells (HMC-1) supernatant on the permeability of human dermal microvascular endothelial cells (HDMECs). Both tryptase and HMC-1 supernatant increased permeability of HDMECs significantly, which was resisted by tryptase inhibitor APC366 and partially reversed by anti-VEGF antibody and SU5614 (catalytic inhibitor of VEGFR). Furthermore, addition of tryptase to HDMECs caused a significant increase of mRNA and protein levels of VEGF and its receptors (Flt-1 and Flk-1) by Real-time RT-PCR and Western blot, respectively. These results strongly suggest an important role of VEGF on the permeability enhancement induced by tryptase, which may lead to novel means of controlling allergic reaction in skin. International Scholarly Research Network 2012-05-15 /pmc/articles/PMC3361195/ /pubmed/22666607 http://dx.doi.org/10.5402/2012/941465 Text en Copyright © 2012 Qianming Bai et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bai, Qianming Li, Xiaobo Wang, Xinhong Xu, Yali Wang, Li Zhang, Qingyong Yin, Lianhua
spellingShingle	Bai, Qianming Li, Xiaobo Wang, Xinhong Xu, Yali Wang, Li Zhang, Qingyong Yin, Lianhua VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
author_facet	Bai, Qianming Li, Xiaobo Wang, Xinhong Xu, Yali Wang, Li Zhang, Qingyong Yin, Lianhua
author_sort	Bai, Qianming
title	VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
title_short	VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
title_full	VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
title_fullStr	VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
title_full_unstemmed	VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
title_sort	vegf is involved in the increase of dermal microvascular permeability induced by tryptase
description	Tryptases are predominantly mast cell-specific serine proteases with pleiotropic biological activities and play a critical role in skin allergic reactions, which are manifested with rapid edema and increases of vascular permeability. The exact mechanisms of mast cell tryptase promoting vascular permeability, however, are unclear and, therefore, we investigated the effect and mechanism of tryptase or human mast cells (HMC-1) supernatant on the permeability of human dermal microvascular endothelial cells (HDMECs). Both tryptase and HMC-1 supernatant increased permeability of HDMECs significantly, which was resisted by tryptase inhibitor APC366 and partially reversed by anti-VEGF antibody and SU5614 (catalytic inhibitor of VEGFR). Furthermore, addition of tryptase to HDMECs caused a significant increase of mRNA and protein levels of VEGF and its receptors (Flt-1 and Flk-1) by Real-time RT-PCR and Western blot, respectively. These results strongly suggest an important role of VEGF on the permeability enhancement induced by tryptase, which may lead to novel means of controlling allergic reaction in skin.
publisher	International Scholarly Research Network
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361195/
_version_	1611532732499755008

VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase

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