Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process

Number of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the...

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Main Authors:	Stojanović, Vesna R., Jovanović, Ivan D., Ugrenović, Sladjana Z., Vasović, Ljiljana P., Živković, Vladimir S., Jocić, Miodrag V., Kundalić, Braca K., Pavlović, Miljana N.
Format:	Online
Language:	English
Published:	The Scientific World Journal 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354636/

id	pubmed-3354636
recordtype	oai_dc
spelling	pubmed-33546362012-05-31 Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process Stojanović, Vesna R. Jovanović, Ivan D. Ugrenović, Sladjana Z. Vasović, Ljiljana P. Živković, Vladimir S. Jocić, Miodrag V. Kundalić, Braca K. Pavlović, Miljana N. Research Article Number of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the percentage of hypertrophic ones in examined human cases during the aging. Material was right kidney's tissue of 30 cadavers obtained during routine autopsies. Cadavers were without previously diagnosed kidney disease, diabetes, hypertension, or any other systemic disease. Tissue specimens were routinely prepared for histological and morphometric analysis. Images of the histological slices were analyzed and captured under 400x magnification with digital camera. Further they were morphometrically and statistically analyzed with ImageJ and NCSS-PASS software. Multiple and linear regression of obtained morphometric parameters showed significant increase of glomerular connective tissue area and percentage. Cluster analysis showed the presence of two types of glomeruli. Second type was characterized with significantly larger size, connective tissue content, and significantly lower cellularity, in relation to the first type. Such glomeruli might be considered as hypertrophic. First type of glomeruli was predominant in younger cases, while second type of glomeruli was predominant in cases older than 55 years. The Scientific World Journal 2012-05-02 /pmc/articles/PMC3354636/ /pubmed/22654637 http://dx.doi.org/10.1100/2012/845046 Text en Copyright © 2012 Vesna R. Stojanović et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Stojanović, Vesna R. Jovanović, Ivan D. Ugrenović, Sladjana Z. Vasović, Ljiljana P. Živković, Vladimir S. Jocić, Miodrag V. Kundalić, Braca K. Pavlović, Miljana N.
spellingShingle	Stojanović, Vesna R. Jovanović, Ivan D. Ugrenović, Sladjana Z. Vasović, Ljiljana P. Živković, Vladimir S. Jocić, Miodrag V. Kundalić, Braca K. Pavlović, Miljana N. Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process
author_facet	Stojanović, Vesna R. Jovanović, Ivan D. Ugrenović, Sladjana Z. Vasović, Ljiljana P. Živković, Vladimir S. Jocić, Miodrag V. Kundalić, Braca K. Pavlović, Miljana N.
author_sort	Stojanović, Vesna R.
title	Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process
title_short	Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process
title_full	Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process
title_fullStr	Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process
title_full_unstemmed	Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process
title_sort	morphometric analysis of nonsclerosed glomeruli size and connective tissue content during the aging process
description	Number of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the percentage of hypertrophic ones in examined human cases during the aging. Material was right kidney's tissue of 30 cadavers obtained during routine autopsies. Cadavers were without previously diagnosed kidney disease, diabetes, hypertension, or any other systemic disease. Tissue specimens were routinely prepared for histological and morphometric analysis. Images of the histological slices were analyzed and captured under 400x magnification with digital camera. Further they were morphometrically and statistically analyzed with ImageJ and NCSS-PASS software. Multiple and linear regression of obtained morphometric parameters showed significant increase of glomerular connective tissue area and percentage. Cluster analysis showed the presence of two types of glomeruli. Second type was characterized with significantly larger size, connective tissue content, and significantly lower cellularity, in relation to the first type. Such glomeruli might be considered as hypertrophic. First type of glomeruli was predominant in younger cases, while second type of glomeruli was predominant in cases older than 55 years.
publisher	The Scientific World Journal
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354636/
_version_	1611530591296028672

Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process

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