MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication
Influenza A virus causes seasonal epidemics and periodic pandemics threatening the health of millions of people each year. Vaccination is an effective strategy for reducing morbidity and mortality, and in the absence of drug resistance, the efficacy of chemoprophylaxis is comparable to that of vacci...
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| Format: | Online |
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Public Library of Science
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351457/ |
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pubmed-3351457 |
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pubmed-33514572012-05-17 MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication Meliopoulos, Victoria A. Andersen, Lauren E. Brooks, Paula Yan, Xiuzhen Bakre, Abhijeet Coleman, J. Keegan Tompkins, S. Mark Tripp, Ralph A. Research Article Influenza A virus causes seasonal epidemics and periodic pandemics threatening the health of millions of people each year. Vaccination is an effective strategy for reducing morbidity and mortality, and in the absence of drug resistance, the efficacy of chemoprophylaxis is comparable to that of vaccines. However, the rapid emergence of drug resistance has emphasized the need for new drug targets. Knowledge of the host cell components required for influenza replication has been an area targeted for disease intervention. In this study, the human protease genes required for influenza virus replication were determined and validated using RNA interference approaches. The genes validated as critical for influenza virus replication were ADAMTS7, CPE, DPP3, MST1, and PRSS12, and pathway analysis showed these genes were in global host cell pathways governing inflammation (NF-κB), cAMP/calcium signaling (CRE/CREB), and apoptosis. Analyses of host microRNAs predicted to govern expression of these genes showed that eight miRNAs regulated gene expression during virus replication. These findings identify unique host genes and microRNAs important for influenza replication providing potential new targets for disease intervention strategies. Public Library of Science 2012-05-14 /pmc/articles/PMC3351457/ /pubmed/22606348 http://dx.doi.org/10.1371/journal.pone.0037169 Text en Meliopoulos et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Meliopoulos, Victoria A. Andersen, Lauren E. Brooks, Paula Yan, Xiuzhen Bakre, Abhijeet Coleman, J. Keegan Tompkins, S. Mark Tripp, Ralph A. |
| spellingShingle |
Meliopoulos, Victoria A. Andersen, Lauren E. Brooks, Paula Yan, Xiuzhen Bakre, Abhijeet Coleman, J. Keegan Tompkins, S. Mark Tripp, Ralph A. MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication |
| author_facet |
Meliopoulos, Victoria A. Andersen, Lauren E. Brooks, Paula Yan, Xiuzhen Bakre, Abhijeet Coleman, J. Keegan Tompkins, S. Mark Tripp, Ralph A. |
| author_sort |
Meliopoulos, Victoria A. |
| title |
MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication |
| title_short |
MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication |
| title_full |
MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication |
| title_fullStr |
MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication |
| title_full_unstemmed |
MicroRNA Regulation of Human Protease Genes Essential for Influenza Virus Replication |
| title_sort |
microrna regulation of human protease genes essential for influenza virus replication |
| description |
Influenza A virus causes seasonal epidemics and periodic pandemics threatening the health of millions of people each year. Vaccination is an effective strategy for reducing morbidity and mortality, and in the absence of drug resistance, the efficacy of chemoprophylaxis is comparable to that of vaccines. However, the rapid emergence of drug resistance has emphasized the need for new drug targets. Knowledge of the host cell components required for influenza replication has been an area targeted for disease intervention. In this study, the human protease genes required for influenza virus replication were determined and validated using RNA interference approaches. The genes validated as critical for influenza virus replication were ADAMTS7, CPE, DPP3, MST1, and PRSS12, and pathway analysis showed these genes were in global host cell pathways governing inflammation (NF-κB), cAMP/calcium signaling (CRE/CREB), and apoptosis. Analyses of host microRNAs predicted to govern expression of these genes showed that eight miRNAs regulated gene expression during virus replication. These findings identify unique host genes and microRNAs important for influenza replication providing potential new targets for disease intervention strategies. |
| publisher |
Public Library of Science |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351457/ |
| _version_ |
1611529369278218240 |