CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription

DNA methylation may regulate gene expression by restricting the access of transcription factors. We have previously demonstrated that GATA-1 regulates the transcription of the CCR3 gene by dynamically interacting with both positively and negatively acting GATA elements of high affinity binding in th...

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Main Authors:	Uhm, Tae Gi, Lee, Seol Kyung, Kim, Byung Soo, Kang, Jin Hyun, Park, Choon-Sik, Rhim, Tai Youn, Chang, Hun Soo, Kim, Do-Jin, Chung, Il Yup
Format:	Online
Language:	English
Published:	Korean Society for Biochemistry and Molecular Biology 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349909/

id	pubmed-3349909
recordtype	oai_dc
spelling	pubmed-33499092012-05-17 CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription Uhm, Tae Gi Lee, Seol Kyung Kim, Byung Soo Kang, Jin Hyun Park, Choon-Sik Rhim, Tai Youn Chang, Hun Soo Kim, Do-Jin Chung, Il Yup Original Article DNA methylation may regulate gene expression by restricting the access of transcription factors. We have previously demonstrated that GATA-1 regulates the transcription of the CCR3 gene by dynamically interacting with both positively and negatively acting GATA elements of high affinity binding in the proximal promoter region including exon 1. Exon 1 has three CpG sites, two of which are positioned at the negatively acting GATA elements. We hypothesized that the methylation of these two CpGs sites might preclude GATA-1 binding to the negatively acting GATA elements and, as a result, increase the availability of GATA-1 to the positively acting GATA element, thereby contributing to an increase in GATA-1-mediated transcription of the gene. To this end, we determined the methylation of the three CpG sites by bisulfate pyrosequencing in peripheral blood eosinophils, cord blood (CB)-derived eosinophils, PBMCs, and cell lines that vary in CCR3 mRNA expression. Our results demonstrated that methylation of CpG sites at the negatively acting GATA elements severely reduced GATA-1 binding and augmented transcription activity in vitro. In agreement, methylation of these CpG sites positively correlated with CCR3 mRNA expression in the primary cells and cell lines examined. Interestingly, methylation patterns of these three CpG sites in CB-derived eosinophils mostly resembled those in peripheral blood eosinophils. These results suggest that methylation of CpG sites at the GATA elements in the regulatory regions fine-tunes CCR3 transcription. Korean Society for Biochemistry and Molecular Biology 2012-04-30 2012-01-05 /pmc/articles/PMC3349909/ /pubmed/22217447 http://dx.doi.org/10.3858/emm.2012.44.4.022 Text en Copyright © 2012 by the Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Uhm, Tae Gi Lee, Seol Kyung Kim, Byung Soo Kang, Jin Hyun Park, Choon-Sik Rhim, Tai Youn Chang, Hun Soo Kim, Do-Jin Chung, Il Yup
spellingShingle	Uhm, Tae Gi Lee, Seol Kyung Kim, Byung Soo Kang, Jin Hyun Park, Choon-Sik Rhim, Tai Youn Chang, Hun Soo Kim, Do-Jin Chung, Il Yup CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription
author_facet	Uhm, Tae Gi Lee, Seol Kyung Kim, Byung Soo Kang, Jin Hyun Park, Choon-Sik Rhim, Tai Youn Chang, Hun Soo Kim, Do-Jin Chung, Il Yup
author_sort	Uhm, Tae Gi
title	CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription
title_short	CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription
title_full	CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription
title_fullStr	CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription
title_full_unstemmed	CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription
title_sort	cpg methylation at gata elements in the regulatory region of ccr3 positively correlates with ccr3 transcription
description	DNA methylation may regulate gene expression by restricting the access of transcription factors. We have previously demonstrated that GATA-1 regulates the transcription of the CCR3 gene by dynamically interacting with both positively and negatively acting GATA elements of high affinity binding in the proximal promoter region including exon 1. Exon 1 has three CpG sites, two of which are positioned at the negatively acting GATA elements. We hypothesized that the methylation of these two CpGs sites might preclude GATA-1 binding to the negatively acting GATA elements and, as a result, increase the availability of GATA-1 to the positively acting GATA element, thereby contributing to an increase in GATA-1-mediated transcription of the gene. To this end, we determined the methylation of the three CpG sites by bisulfate pyrosequencing in peripheral blood eosinophils, cord blood (CB)-derived eosinophils, PBMCs, and cell lines that vary in CCR3 mRNA expression. Our results demonstrated that methylation of CpG sites at the negatively acting GATA elements severely reduced GATA-1 binding and augmented transcription activity in vitro. In agreement, methylation of these CpG sites positively correlated with CCR3 mRNA expression in the primary cells and cell lines examined. Interestingly, methylation patterns of these three CpG sites in CB-derived eosinophils mostly resembled those in peripheral blood eosinophils. These results suggest that methylation of CpG sites at the GATA elements in the regulatory regions fine-tunes CCR3 transcription.
publisher	Korean Society for Biochemistry and Molecular Biology
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349909/
_version_	1611528855720296448

CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription

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