2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC
Stimulation of CD95 (APO–1/Fas) leads to apoptosis induction in multicellular organisms. CD95–mediated apoptosis starts with the formation of the protein complex at the receptor CD95 (APO–1/Fas), which was named DISC (death–inducing signaling complex). In this work, the composition of the CD95 DISC...
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| Format: | Online |
| Language: | English |
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A.I. Gordeyev
2010
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347551/ |
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pubmed-33475512012-05-30 2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC Riess, D. Lavrik, I. Research Article Stimulation of CD95 (APO–1/Fas) leads to apoptosis induction in multicellular organisms. CD95–mediated apoptosis starts with the formation of the protein complex at the receptor CD95 (APO–1/Fas), which was named DISC (death–inducing signaling complex). In this work, the composition of the CD95 DISC in two different cell types was analyzed using proteomics approaches. Using 2D gels, the composition of the CD95 DISC was analyzed in the so–called Type I and Type II cells, which are characterized by different kinetics of apoptosis. The detailed analysis of the CD95 DISC performed by 2D gels demonstrated that, besides the well–established components of the CD95 DISC, which are present in both cell types (CD95, FADD and procaspase–8), there are a number of differential spots detected at the CD95 DISC of Type I versus Type II cells. Taken together, this work demonstrates the differential composition of the CD95 DISC of Type I versus Type II cells. A.I. Gordeyev 2010-07 /pmc/articles/PMC3347551/ /pubmed/22649647 Text en Copyright © 2010 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Riess, D. Lavrik, I. |
| spellingShingle |
Riess, D. Lavrik, I. 2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC |
| author_facet |
Riess, D. Lavrik, I. |
| author_sort |
Riess, D. |
| title |
2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC |
| title_short |
2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC |
| title_full |
2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC |
| title_fullStr |
2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC |
| title_full_unstemmed |
2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC |
| title_sort |
2d-gel electrophoresis as a tool to investigate the composition of cd95 disc |
| description |
Stimulation of CD95 (APO–1/Fas) leads to apoptosis induction in multicellular organisms.
CD95–mediated apoptosis starts with the formation of the protein complex at the receptor
CD95 (APO–1/Fas), which was named DISC (death–inducing signaling complex). In this
work, the composition of the CD95 DISC in two different cell types was analyzed using
proteomics approaches. Using 2D gels, the composition of the CD95 DISC was analyzed in the
so–called Type I and Type II cells, which are characterized by different kinetics of
apoptosis. The detailed analysis of the CD95 DISC performed by 2D gels demonstrated that,
besides the well–established components of the CD95 DISC, which are present in both cell
types (CD95, FADD and procaspase–8), there are a number of differential spots detected at
the CD95 DISC of Type I versus Type II cells. Taken together, this work
demonstrates the differential composition of the CD95 DISC of Type I versus
Type II cells.
|
| publisher |
A.I. Gordeyev |
| publishDate |
2010 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347551/ |
| _version_ |
1611528140787548160 |