Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography

Micro-scale, non-invasive, three-dimensional cross-sectional imaging of protein crystals was successfully accomplished using ultra-high resolution optical coherence tomography (UHR-OCT) with low noise, Gaussian like supercontinuum. This technique facilitated visualization of protein crystals even th...

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Main Authors:	Nishizawa, Norihiko, Ishida, Shutaro, Hirose, Mika, Sugiyama, Shigeru, Inoue, Tsuyoshi, Mori, Yusuke, Itoh, Kazuyoshi, Matsumura, Hiroyoshi
Format:	Online
Language:	English
Published:	Optical Society of America 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3345802/

id	pubmed-3345802
recordtype	oai_dc
spelling	pubmed-33458022012-05-09 Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography Nishizawa, Norihiko Ishida, Shutaro Hirose, Mika Sugiyama, Shigeru Inoue, Tsuyoshi Mori, Yusuke Itoh, Kazuyoshi Matsumura, Hiroyoshi Optical Coherence Tomography Micro-scale, non-invasive, three-dimensional cross-sectional imaging of protein crystals was successfully accomplished using ultra-high resolution optical coherence tomography (UHR-OCT) with low noise, Gaussian like supercontinuum. This technique facilitated visualization of protein crystals even those in medium that also contained substantial amounts of precipitates. We found the enhancement of the scattered signal from protein crystal by inclusion of agarose gel in the crystallization medium. Crystals of a protein and a salt in the same sample when visualized by UHR-OCT showed distinct physical characteristics, suggesting that protein and salt crystals may, in general, be distinguishable by UHR-OCT. UHR-OCT is a nondestructive and rapid method, which should therefore find use in automated systems designed to visualize crystals. Optical Society of America 2012-03-15 /pmc/articles/PMC3345802/ /pubmed/22574261 http://dx.doi.org/10.1364/BOE.3.000735 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Nishizawa, Norihiko Ishida, Shutaro Hirose, Mika Sugiyama, Shigeru Inoue, Tsuyoshi Mori, Yusuke Itoh, Kazuyoshi Matsumura, Hiroyoshi
spellingShingle	Nishizawa, Norihiko Ishida, Shutaro Hirose, Mika Sugiyama, Shigeru Inoue, Tsuyoshi Mori, Yusuke Itoh, Kazuyoshi Matsumura, Hiroyoshi Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
author_facet	Nishizawa, Norihiko Ishida, Shutaro Hirose, Mika Sugiyama, Shigeru Inoue, Tsuyoshi Mori, Yusuke Itoh, Kazuyoshi Matsumura, Hiroyoshi
author_sort	Nishizawa, Norihiko
title	Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
title_short	Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
title_full	Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
title_fullStr	Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
title_full_unstemmed	Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
title_sort	three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
description	Micro-scale, non-invasive, three-dimensional cross-sectional imaging of protein crystals was successfully accomplished using ultra-high resolution optical coherence tomography (UHR-OCT) with low noise, Gaussian like supercontinuum. This technique facilitated visualization of protein crystals even those in medium that also contained substantial amounts of precipitates. We found the enhancement of the scattered signal from protein crystal by inclusion of agarose gel in the crystallization medium. Crystals of a protein and a salt in the same sample when visualized by UHR-OCT showed distinct physical characteristics, suggesting that protein and salt crystals may, in general, be distinguishable by UHR-OCT. UHR-OCT is a nondestructive and rapid method, which should therefore find use in automated systems designed to visualize crystals.
publisher	Optical Society of America
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3345802/
_version_	1611527796214988800

Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography

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