Refractive errors and corrections for OCT images in an inflated lung phantom
Visualization and correct assessment of alveolar volume via intact lung imaging is important to study and assess respiratory mechanics. Optical Coherence Tomography (OCT), a real-time imaging technique based on near-infrared interferometry, can image several layers of distal alveoli in intact, ex vi...
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Optical Society of America
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342185/ |
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pubmed-33421852012-05-07 Refractive errors and corrections for OCT images in an inflated lung phantom Golabchi, Ali Faust, J. Golabchi, F. N. Brooks, D. H. Gouldstone, A. DiMarzio, C. A. Optical Coherence Tomography Visualization and correct assessment of alveolar volume via intact lung imaging is important to study and assess respiratory mechanics. Optical Coherence Tomography (OCT), a real-time imaging technique based on near-infrared interferometry, can image several layers of distal alveoli in intact, ex vivo lung tissue. However optical effects associated with heterogeneity of lung tissue, including the refraction caused by air-tissue interfaces along alveoli and duct walls, and changes in speed of light as it travels through the tissue, result in inaccurate measurement of alveolar volume. Experimentally such errors have been difficult to analyze because of lack of ’ground truth,’ as the lung has a unique microstructure of liquid-coated thin walls surrounding relatively large airspaces, which is difficult to model with cellular foams. In addition, both lung and foams contain airspaces of highly irregular shape, further complicating quantitative measurement of optical artifacts and correction. To address this we have adapted the Bragg-Nye bubble raft, a crystalline two-dimensional arrangement of elements similar in geometry to alveoli (up to several hundred μm in diameter with thin walls) as an inflated lung phantom in order to understand, analyze and correct these errors. By applying exact optical ray tracing on OCT images of the bubble raft, the errors are predicted and corrected. The results are validated by imaging the bubble raft with OCT from one edge and with a charged coupled device (CCD) camera in transillumination from top, providing ground truth for the OCT. Optical Society of America 2012-04-25 /pmc/articles/PMC3342185/ /pubmed/22567599 http://dx.doi.org/10.1364/BOE.3.001101 Text en © 2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Golabchi, Ali Faust, J. Golabchi, F. N. Brooks, D. H. Gouldstone, A. DiMarzio, C. A. |
| spellingShingle |
Golabchi, Ali Faust, J. Golabchi, F. N. Brooks, D. H. Gouldstone, A. DiMarzio, C. A. Refractive errors and corrections for OCT images in an inflated lung phantom |
| author_facet |
Golabchi, Ali Faust, J. Golabchi, F. N. Brooks, D. H. Gouldstone, A. DiMarzio, C. A. |
| author_sort |
Golabchi, Ali |
| title |
Refractive errors and corrections for OCT images in an inflated lung phantom |
| title_short |
Refractive errors and corrections for OCT images in an inflated lung phantom |
| title_full |
Refractive errors and corrections for OCT images in an inflated lung phantom |
| title_fullStr |
Refractive errors and corrections for OCT images in an inflated lung phantom |
| title_full_unstemmed |
Refractive errors and corrections for OCT images in an inflated lung phantom |
| title_sort |
refractive errors and corrections for oct images in an inflated lung phantom |
| description |
Visualization and correct assessment of alveolar volume via intact lung imaging is important to study and assess respiratory mechanics. Optical Coherence Tomography (OCT), a real-time imaging technique based on near-infrared interferometry, can image several layers of distal alveoli in intact, ex vivo lung tissue. However optical effects associated with heterogeneity of lung tissue, including the refraction caused by air-tissue interfaces along alveoli and duct walls, and changes in speed of light as it travels through the tissue, result in inaccurate measurement of alveolar volume. Experimentally such errors have been difficult to analyze because of lack of ’ground truth,’ as the lung has a unique microstructure of liquid-coated thin walls surrounding relatively large airspaces, which is difficult to model with cellular foams. In addition, both lung and foams contain airspaces of highly irregular shape, further complicating quantitative measurement of optical artifacts and correction. To address this we have adapted the Bragg-Nye bubble raft, a crystalline two-dimensional arrangement of elements similar in geometry to alveoli (up to several hundred μm in diameter with thin walls) as an inflated lung phantom in order to understand, analyze and correct these errors. By applying exact optical ray tracing on OCT images of the bubble raft, the errors are predicted and corrected. The results are validated by imaging the bubble raft with OCT from one edge and with a charged coupled device (CCD) camera in transillumination from top, providing ground truth for the OCT. |
| publisher |
Optical Society of America |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342185/ |
| _version_ |
1611526496435830784 |