Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid
Carnosic acid (CA) is a diterpene compound exhibiting antioxidative, anticancer, anti-angiogenic, anti-inflammatory, anti-metabolic disorder, and hepatoprotective and neuroprotective activities. In this study, the effect of CA on various skin inflammatory responses and its inhibitory mechanism were...
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| Format: | Online |
| Language: | English |
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Hindawi Publishing Corporation
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3337681/ |
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pubmed-3337681 |
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pubmed-33376812012-05-10 Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid Oh, Jueun Yu, Tao Choi, Soo Jeong Yang, Yanyan Baek, Heung Soo An, Soon Ae Kwon, Lee Kyoung Kim, Jinsol Rho, Ho Sik Shin, Song Seok Choi, Wahn Soo Hong, Sungyoul Cho, Jae Youl Research Article Carnosic acid (CA) is a diterpene compound exhibiting antioxidative, anticancer, anti-angiogenic, anti-inflammatory, anti-metabolic disorder, and hepatoprotective and neuroprotective activities. In this study, the effect of CA on various skin inflammatory responses and its inhibitory mechanism were examined. CA strongly suppressed the production of IL-6, IL-8, and MCP-1 from keratinocyte HaCaT cells stimulated with sodium lauryl sulfate (SLS) and retinoic acid (RA). In addition, CA blocked the release of nitric oxide (NO), tumor necrosis factor (TNF)-α, and prostaglandin E2 (PGE2) from RAW264.7 cells activated by the toll-like receptor (TLR)-2 ligands, Gram-positive bacterium-derived peptidoglycan (PGN) and pam3CSK, and the TLR4 ligand, Gram-negative bacterium-derived lipopolysaccharide (LPS). CA arrested the growth of dermatitis-inducing Gram-positive and Gram-negative microorganisms such Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus aureus. CA also blocked the nuclear translocation of nuclear factor (NF)-κB and its upstream signaling including Syk/Src, phosphoinositide 3-kinase (PI3K), Akt, inhibitor of κBα (IκBα) kinase (IKK), and IκBα for NF-κB activation. Kinase assays revealed that Syk could be direct enzymatic target of CA in its anti-inflammatory action. Therefore, our data strongly suggest the potential of CA as an anti-inflammatory drug against skin inflammatory responses with Src/NF-κB inhibitory properties. Hindawi Publishing Corporation 2012 2012-04-10 /pmc/articles/PMC3337681/ /pubmed/22577255 http://dx.doi.org/10.1155/2012/781375 Text en Copyright © 2012 Jueun Oh et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Oh, Jueun Yu, Tao Choi, Soo Jeong Yang, Yanyan Baek, Heung Soo An, Soon Ae Kwon, Lee Kyoung Kim, Jinsol Rho, Ho Sik Shin, Song Seok Choi, Wahn Soo Hong, Sungyoul Cho, Jae Youl |
| spellingShingle |
Oh, Jueun Yu, Tao Choi, Soo Jeong Yang, Yanyan Baek, Heung Soo An, Soon Ae Kwon, Lee Kyoung Kim, Jinsol Rho, Ho Sik Shin, Song Seok Choi, Wahn Soo Hong, Sungyoul Cho, Jae Youl Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid |
| author_facet |
Oh, Jueun Yu, Tao Choi, Soo Jeong Yang, Yanyan Baek, Heung Soo An, Soon Ae Kwon, Lee Kyoung Kim, Jinsol Rho, Ho Sik Shin, Song Seok Choi, Wahn Soo Hong, Sungyoul Cho, Jae Youl |
| author_sort |
Oh, Jueun |
| title |
Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid |
| title_short |
Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid |
| title_full |
Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid |
| title_fullStr |
Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid |
| title_full_unstemmed |
Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid |
| title_sort |
syk/src pathway-targeted inhibition of skin inflammatory responses by carnosic acid |
| description |
Carnosic acid (CA) is a diterpene compound exhibiting antioxidative, anticancer, anti-angiogenic, anti-inflammatory, anti-metabolic disorder, and hepatoprotective and neuroprotective activities. In this study, the effect of CA on various skin inflammatory responses and its inhibitory mechanism were examined. CA strongly suppressed the production of IL-6, IL-8, and MCP-1 from keratinocyte HaCaT cells stimulated with sodium lauryl sulfate (SLS) and retinoic acid (RA). In addition, CA blocked the release of nitric oxide (NO), tumor necrosis factor (TNF)-α, and prostaglandin E2 (PGE2) from RAW264.7 cells activated by the toll-like receptor (TLR)-2 ligands, Gram-positive bacterium-derived peptidoglycan (PGN) and pam3CSK, and the TLR4 ligand, Gram-negative bacterium-derived lipopolysaccharide (LPS). CA arrested the growth of dermatitis-inducing Gram-positive and Gram-negative microorganisms such Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus aureus. CA also blocked the nuclear translocation of nuclear factor (NF)-κB and its upstream signaling including Syk/Src, phosphoinositide 3-kinase (PI3K), Akt, inhibitor of κBα (IκBα) kinase (IKK), and IκBα for NF-κB activation. Kinase assays revealed that Syk could be direct enzymatic target of CA in its anti-inflammatory action. Therefore, our data strongly suggest the potential of CA as an anti-inflammatory drug against skin inflammatory responses with Src/NF-κB inhibitory properties. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3337681/ |
| _version_ |
1611525109586067456 |