Modulation of Wnt5a Expression by Periodontopathic Bacteria
Wingless proteins, termed Wnt, are involved in embryonic development, blood cell differentiation, and tumorigenesis. In mammalian hematopoiesis, Wnt signaling is essential for stem-cell homeostasis and lymphocyte differentiation. Recent studies have suggested that these molecules are associated with...
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| Format: | Online |
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Public Library of Science
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317782/ |
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pubmed-33177822012-04-06 Modulation of Wnt5a Expression by Periodontopathic Bacteria Nanbara, Hiromi Wara-aswapati, Nawarat Nagasawa, Toshiyuki Yoshida, Yasuhiro Yashiro, Reiko Bando, Yukiko Kobayashi, Hiroaki Khongcharoensuk, Janjura Hormdee, Doosadee Pitiphat, Waranuch Boch, Jason A. Izumi, Yuichi Research Article Wingless proteins, termed Wnt, are involved in embryonic development, blood cell differentiation, and tumorigenesis. In mammalian hematopoiesis, Wnt signaling is essential for stem-cell homeostasis and lymphocyte differentiation. Recent studies have suggested that these molecules are associated with cardiovascular diseases, rheumatoid arthritis, and osteoarthritis. Furthermore, Wnt5a signaling is essential for the general inflammatory response of human macrophages. Periodontitis is a chronic inflammatory disease caused by gram-negative periodontopathic bacteria and the resultant host immune response. Periodontitis is characterized by loss of tooth-supporting structures and alveolar bone resorption. There have been no previous reports on Wnt5a expression in periodontitis tissue, and only few study reported the molecular mechanisms of Wnt5a expression in LPS-stimulated monocytic cells. Using RT-PCR, we demonstrated that Wnt5a mRNA expression was up-regulated in chronic periodontitis tissue as compared to healthy control tissue. P. gingivalis LPS induced Wnt5a mRNA in the human monocytic cell line THP-1 with a peak at 4 hrs after stimulation. P. gingivalis LPS induced higher up-regulation of Wnt5a mRNA than E. coli LPS. The LPS receptors TLR2 and TLR4 were equally expressed on the surface of THP-1 cells. P. gingivalis LPS induced IκBα degradation and was able to increase the NF-κB binding activity to DNA. P. gingivalis LPS-induced Wnt5a expression was inhibited by NF-κB inhibitors, suggesting NF-κB involvement. Furthermore, IFN-γ synergistically enhanced the P. gingivalis LPS-induced production of Wnt5a. Pharmacological investigation and siRNA experiments showed that STAT1 was important for P. gingivalis LPS-induced Wnt5a expression. These results suggest that the modulation of Wnt5a expression by P. gingivalis may play an important role in the periodontal inflammatory process and serve a target for the development of new therapies. Public Library of Science 2012-04-02 /pmc/articles/PMC3317782/ /pubmed/22485170 http://dx.doi.org/10.1371/journal.pone.0034434 Text en Nanbara et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Nanbara, Hiromi Wara-aswapati, Nawarat Nagasawa, Toshiyuki Yoshida, Yasuhiro Yashiro, Reiko Bando, Yukiko Kobayashi, Hiroaki Khongcharoensuk, Janjura Hormdee, Doosadee Pitiphat, Waranuch Boch, Jason A. Izumi, Yuichi |
| spellingShingle |
Nanbara, Hiromi Wara-aswapati, Nawarat Nagasawa, Toshiyuki Yoshida, Yasuhiro Yashiro, Reiko Bando, Yukiko Kobayashi, Hiroaki Khongcharoensuk, Janjura Hormdee, Doosadee Pitiphat, Waranuch Boch, Jason A. Izumi, Yuichi Modulation of Wnt5a Expression by Periodontopathic Bacteria |
| author_facet |
Nanbara, Hiromi Wara-aswapati, Nawarat Nagasawa, Toshiyuki Yoshida, Yasuhiro Yashiro, Reiko Bando, Yukiko Kobayashi, Hiroaki Khongcharoensuk, Janjura Hormdee, Doosadee Pitiphat, Waranuch Boch, Jason A. Izumi, Yuichi |
| author_sort |
Nanbara, Hiromi |
| title |
Modulation of Wnt5a Expression by Periodontopathic Bacteria |
| title_short |
Modulation of Wnt5a Expression by Periodontopathic Bacteria |
| title_full |
Modulation of Wnt5a Expression by Periodontopathic Bacteria |
| title_fullStr |
Modulation of Wnt5a Expression by Periodontopathic Bacteria |
| title_full_unstemmed |
Modulation of Wnt5a Expression by Periodontopathic Bacteria |
| title_sort |
modulation of wnt5a expression by periodontopathic bacteria |
| description |
Wingless proteins, termed Wnt, are involved in embryonic development, blood cell differentiation, and tumorigenesis. In mammalian hematopoiesis, Wnt signaling is essential for stem-cell homeostasis and lymphocyte differentiation. Recent studies have suggested that these molecules are associated with cardiovascular diseases, rheumatoid arthritis, and osteoarthritis. Furthermore, Wnt5a signaling is essential for the general inflammatory response of human macrophages. Periodontitis is a chronic inflammatory disease caused by gram-negative periodontopathic bacteria and the resultant host immune response. Periodontitis is characterized by loss of tooth-supporting structures and alveolar bone resorption. There have been no previous reports on Wnt5a expression in periodontitis tissue, and only few study reported the molecular mechanisms of Wnt5a expression in LPS-stimulated monocytic cells. Using RT-PCR, we demonstrated that Wnt5a mRNA expression was up-regulated in chronic periodontitis tissue as compared to healthy control tissue. P. gingivalis LPS induced Wnt5a mRNA in the human monocytic cell line THP-1 with a peak at 4 hrs after stimulation. P. gingivalis LPS induced higher up-regulation of Wnt5a mRNA than E. coli LPS. The LPS receptors TLR2 and TLR4 were equally expressed on the surface of THP-1 cells. P. gingivalis LPS induced IκBα degradation and was able to increase the NF-κB binding activity to DNA. P. gingivalis LPS-induced Wnt5a expression was inhibited by NF-κB inhibitors, suggesting NF-κB involvement. Furthermore, IFN-γ synergistically enhanced the P. gingivalis LPS-induced production of Wnt5a. Pharmacological investigation and siRNA experiments showed that STAT1 was important for P. gingivalis LPS-induced Wnt5a expression. These results suggest that the modulation of Wnt5a expression by P. gingivalis may play an important role in the periodontal inflammatory process and serve a target for the development of new therapies. |
| publisher |
Public Library of Science |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317782/ |
| _version_ |
1611518670450720768 |