The Effect of Osmolytes on Protein Fibrillation

Osmolytes are small molecules that are exploited by cells as a protective system against stress conditions. They favour compact protein states which makes them stabilize globular proteins in vitro and promote folding. Conversely, this preference for compact states promotes aggregation of unstructure...

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Main Authors:	Macchi, Francesca, Eisenkolb, Maike, Kiefer, Hans, Otzen, Daniel E.
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317744/

id	pubmed-3317744
recordtype	oai_dc
spelling	pubmed-33177442012-04-09 The Effect of Osmolytes on Protein Fibrillation Macchi, Francesca Eisenkolb, Maike Kiefer, Hans Otzen, Daniel E. Article Osmolytes are small molecules that are exploited by cells as a protective system against stress conditions. They favour compact protein states which makes them stabilize globular proteins in vitro and promote folding. Conversely, this preference for compact states promotes aggregation of unstructured proteins. Here we combine a brief review of the effect of osmolytes on protein fibrillation with a report of the effect of osmolytes on the unstructured peptide hormone glucagon. Our results show that osmolytes either accelerate the fibrillation kinetics or leave them unaffected, with the exception of the osmolyte taurine. Furthermore, the osmolytes that affected the shape of the fibrillation time profile led to fibrils with different structure as revealed by CD. The structural changes induced by Pro, Ser and choline-O-sulfate could be due to specific osmolytes binding to the peptides, stabilizing an otherwise labile fibrillation intermediate. Molecular Diversity Preservation International (MDPI) 2012-03-21 /pmc/articles/PMC3317744/ /pubmed/22489184 http://dx.doi.org/10.3390/ijms13033801 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Macchi, Francesca Eisenkolb, Maike Kiefer, Hans Otzen, Daniel E.
spellingShingle	Macchi, Francesca Eisenkolb, Maike Kiefer, Hans Otzen, Daniel E. The Effect of Osmolytes on Protein Fibrillation
author_facet	Macchi, Francesca Eisenkolb, Maike Kiefer, Hans Otzen, Daniel E.
author_sort	Macchi, Francesca
title	The Effect of Osmolytes on Protein Fibrillation
title_short	The Effect of Osmolytes on Protein Fibrillation
title_full	The Effect of Osmolytes on Protein Fibrillation
title_fullStr	The Effect of Osmolytes on Protein Fibrillation
title_full_unstemmed	The Effect of Osmolytes on Protein Fibrillation
title_sort	effect of osmolytes on protein fibrillation
description	Osmolytes are small molecules that are exploited by cells as a protective system against stress conditions. They favour compact protein states which makes them stabilize globular proteins in vitro and promote folding. Conversely, this preference for compact states promotes aggregation of unstructured proteins. Here we combine a brief review of the effect of osmolytes on protein fibrillation with a report of the effect of osmolytes on the unstructured peptide hormone glucagon. Our results show that osmolytes either accelerate the fibrillation kinetics or leave them unaffected, with the exception of the osmolyte taurine. Furthermore, the osmolytes that affected the shape of the fibrillation time profile led to fibrils with different structure as revealed by CD. The structural changes induced by Pro, Ser and choline-O-sulfate could be due to specific osmolytes binding to the peptides, stabilizing an otherwise labile fibrillation intermediate.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317744/
_version_	1611518649446694912

The Effect of Osmolytes on Protein Fibrillation

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