B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis

Hepatic resistance to Leishmania donovani infection in mice is associated with the development of granulomas, in which a variety of lymphoid and non-lymphoid populations accumulate. Although previous studies have identified B cells in hepatic granulomas and functional studies in B cell-deficient mic...

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Main Authors:	Moore, John W. J., Beattie, Lynette, Dalton, Jane E., Owens, Benjamin M. J., Maroof, Asher, Coles, Mark C., Kaye, Paul M.
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316612/

id	pubmed-3316612
recordtype	oai_dc
spelling	pubmed-33166122012-04-04 B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis Moore, John W. J. Beattie, Lynette Dalton, Jane E. Owens, Benjamin M. J. Maroof, Asher Coles, Mark C. Kaye, Paul M. Research Article Hepatic resistance to Leishmania donovani infection in mice is associated with the development of granulomas, in which a variety of lymphoid and non-lymphoid populations accumulate. Although previous studies have identified B cells in hepatic granulomas and functional studies in B cell-deficient mice have suggested a role for B cells in the control of experimental visceral leishmaniasis, little is known about the behaviour of B cells in the granuloma microenvironment. Here, we first compared the hepatic B cell population in infected mice, where ≈60% of B cells are located within granulomas, with that of naïve mice. In infected mice, there was a small increase in mIgMlomIgD+ mature B2 cells, but no enrichment of B cells with regulatory phenotype or function compared to the naïve hepatic B cell population, as assessed by CD1d and CD5 expression and by IL-10 production. Using 2-photon microscopy to quantify the entire intra-granuloma B cell population, in conjunction with the adoptive transfer of polyclonal and HEL-specific BCR-transgenic B cells isolated from L. donovani-infected mice, we demonstrated that B cells accumulate in granulomas over time in an antigen-independent manner. Intra-vital dynamic imaging was used to demonstrate that within the polyclonal B cell population obtained from L. donovani-infected mice, the frequency of B cells that made multiple long contacts with endogenous T cells was greater than that observed using HEL-specific B cells obtained from the same inflammatory environment. These data indicate, therefore, that a subset of this polyclonal B cell population is capable of making cognate interactions with T cells within this unique environment, and provide the first insights into the dynamics of B cells within an inflammatory site. Public Library of Science 2012-03-30 /pmc/articles/PMC3316612/ /pubmed/22479545 http://dx.doi.org/10.1371/journal.pone.0034143 Text en © 2012 Moore et al This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Moore, John W. J. Beattie, Lynette Dalton, Jane E. Owens, Benjamin M. J. Maroof, Asher Coles, Mark C. Kaye, Paul M.
spellingShingle	Moore, John W. J. Beattie, Lynette Dalton, Jane E. Owens, Benjamin M. J. Maroof, Asher Coles, Mark C. Kaye, Paul M. B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis
author_facet	Moore, John W. J. Beattie, Lynette Dalton, Jane E. Owens, Benjamin M. J. Maroof, Asher Coles, Mark C. Kaye, Paul M.
author_sort	Moore, John W. J.
title	B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis
title_short	B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis
title_full	B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis
title_fullStr	B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis
title_full_unstemmed	B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis
title_sort	b cell: t cell interactions occur within hepatic granulomas during experimental visceral leishmaniasis
description	Hepatic resistance to Leishmania donovani infection in mice is associated with the development of granulomas, in which a variety of lymphoid and non-lymphoid populations accumulate. Although previous studies have identified B cells in hepatic granulomas and functional studies in B cell-deficient mice have suggested a role for B cells in the control of experimental visceral leishmaniasis, little is known about the behaviour of B cells in the granuloma microenvironment. Here, we first compared the hepatic B cell population in infected mice, where ≈60% of B cells are located within granulomas, with that of naïve mice. In infected mice, there was a small increase in mIgMlomIgD+ mature B2 cells, but no enrichment of B cells with regulatory phenotype or function compared to the naïve hepatic B cell population, as assessed by CD1d and CD5 expression and by IL-10 production. Using 2-photon microscopy to quantify the entire intra-granuloma B cell population, in conjunction with the adoptive transfer of polyclonal and HEL-specific BCR-transgenic B cells isolated from L. donovani-infected mice, we demonstrated that B cells accumulate in granulomas over time in an antigen-independent manner. Intra-vital dynamic imaging was used to demonstrate that within the polyclonal B cell population obtained from L. donovani-infected mice, the frequency of B cells that made multiple long contacts with endogenous T cells was greater than that observed using HEL-specific B cells obtained from the same inflammatory environment. These data indicate, therefore, that a subset of this polyclonal B cell population is capable of making cognate interactions with T cells within this unique environment, and provide the first insights into the dynamics of B cells within an inflammatory site.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316612/
_version_	1611518223676604416

B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis

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