A novel, simple, and sensitive colorimetric method to determine aromatic amino acid aminotransferase activity using the Salkowski reagent
This study describes the development of a new colorimetric assay to determine aromatic amino acid aminotransferase (ArAT) activity. The assay is based on the transamination of l-tryptophan in the presence of 2-oxoglutarate, which yields indole-3-pyruvate (IPyA). The amount of IPyA formed was quantif...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Springer Netherlands
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297745/ |