Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica

Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica

The primary transcript of rRNA genes is a large pre rRNA which is precisely processed to release the mature rRNAs. The 5′-external transcribed spacer (ETS) of rRNA genes contains important sites for pre rRNA processing. Once the processing is accomplished the ETS is rapidly degraded. We show that in...

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Main Authors: Gupta, Abhishek Kumar, Panigrahi, Sunil Kumar, Bhattacharya, Alok, Bhattacharya, Sudha
Format: Online
Language:English
Published: Nature Publishing Group 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294279/
id pubmed-3294279
recordtype oai_dc
spelling pubmed-32942792012-03-06 Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica Gupta, Abhishek Kumar Panigrahi, Sunil Kumar Bhattacharya, Alok Bhattacharya, Sudha Article The primary transcript of rRNA genes is a large pre rRNA which is precisely processed to release the mature rRNAs. The 5′-external transcribed spacer (ETS) of rRNA genes contains important sites for pre rRNA processing. Once the processing is accomplished the ETS is rapidly degraded. We show that in growth-stressed cells of the human parasitic protist Entamoeba histolytica the A'-A0 sub-fragment of the 5′-ETS accumulates to high levels as a family of RNA molecules of size 666 to 912 nt. These etsRNAs are circular in vivo and can spontaneously self-circularize in vitro. The accumulation of etsRNAs is accompanied by accumulation of unprocessed pre rRNA, indicating a possible role of etsRNAs in inhibition of processing during growth stress. Our data shows for the first time that processed etsRNA is not a mere by-product destined for degradation but is stabilized by circularization and could play a regulatory role as noncoding RNA. Nature Publishing Group 2012-03-06 /pmc/articles/PMC3294279/ /pubmed/22396851 http://dx.doi.org/10.1038/srep00303 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Gupta, Abhishek Kumar
Panigrahi, Sunil Kumar
Bhattacharya, Alok
Bhattacharya, Sudha
spellingShingle Gupta, Abhishek Kumar
Panigrahi, Sunil Kumar
Bhattacharya, Alok
Bhattacharya, Sudha
Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica
author_facet Gupta, Abhishek Kumar
Panigrahi, Sunil Kumar
Bhattacharya, Alok
Bhattacharya, Sudha
author_sort Gupta, Abhishek Kumar
title Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica
title_short Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica
title_full Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica
title_fullStr Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica
title_full_unstemmed Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica
title_sort self-circularizing 5′-ets rnas accumulate along with unprocessed pre ribosomal rnas in growth-stressed entamoeba histolytica
description The primary transcript of rRNA genes is a large pre rRNA which is precisely processed to release the mature rRNAs. The 5′-external transcribed spacer (ETS) of rRNA genes contains important sites for pre rRNA processing. Once the processing is accomplished the ETS is rapidly degraded. We show that in growth-stressed cells of the human parasitic protist Entamoeba histolytica the A'-A0 sub-fragment of the 5′-ETS accumulates to high levels as a family of RNA molecules of size 666 to 912 nt. These etsRNAs are circular in vivo and can spontaneously self-circularize in vitro. The accumulation of etsRNAs is accompanied by accumulation of unprocessed pre rRNA, indicating a possible role of etsRNAs in inhibition of processing during growth stress. Our data shows for the first time that processed etsRNA is not a mere by-product destined for degradation but is stabilized by circularization and could play a regulatory role as noncoding RNA.
publisher Nature Publishing Group
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294279/
_version_ 1611510587826634752

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