In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)

It has been hypothesized that blood-brain barrier (BBB) dysfunction in Angiostrongylus cantonensis infection might be due to the apoptosis of the hosts' BBB cells. Here, we evaluated this hypothesis through several methods, all based on an in vitro mouse BBB model consisting of primary culture...

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Main Authors:	Hu, Xin, Li, Jiang-Hui, Lan, Lan, Wu, Fei-Fei, Zhang, Er-Peng, Song, Zeng-Mei, Huang, Hui-Cong, Luo, Fang-Jun, Pan, Chang-Wang, Tan, Feng
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290544/

id	pubmed-3290544
recordtype	oai_dc
spelling	pubmed-32905442012-03-05 In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB) Hu, Xin Li, Jiang-Hui Lan, Lan Wu, Fei-Fei Zhang, Er-Peng Song, Zeng-Mei Huang, Hui-Cong Luo, Fang-Jun Pan, Chang-Wang Tan, Feng Research Article It has been hypothesized that blood-brain barrier (BBB) dysfunction in Angiostrongylus cantonensis infection might be due to the apoptosis of the hosts' BBB cells. Here, we evaluated this hypothesis through several methods, all based on an in vitro mouse BBB model consisting of primary culture brain microvascular endothelial cells (BMECs) and brain astrocytic cells (BACs). In the present study, a four-hour percolation and HRP permeability experiment showed that A. cantonensis larvae extracts can increase the permeability of the BBB. Apoptosis among BMECs and BACs after exposure to larvae extracts was monitored by TUNEL and annexin-V-FITC/PI double staining. A. cantonensis larvae extracts were found to induce apoptosis in both BMECs and BACs. For this reason, we concluded that the induction of apoptosis might participate in the BBB dysfunction observed during angiostrongyliasis. Improved fundamental understanding of how A. cantonensis induces apoptosis may lead to new approaches to the treatment or prevention of this parasitic disease. Public Library of Science 2012-02-29 /pmc/articles/PMC3290544/ /pubmed/22393387 http://dx.doi.org/10.1371/journal.pone.0032161 Text en Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hu, Xin Li, Jiang-Hui Lan, Lan Wu, Fei-Fei Zhang, Er-Peng Song, Zeng-Mei Huang, Hui-Cong Luo, Fang-Jun Pan, Chang-Wang Tan, Feng
spellingShingle	Hu, Xin Li, Jiang-Hui Lan, Lan Wu, Fei-Fei Zhang, Er-Peng Song, Zeng-Mei Huang, Hui-Cong Luo, Fang-Jun Pan, Chang-Wang Tan, Feng In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)
author_facet	Hu, Xin Li, Jiang-Hui Lan, Lan Wu, Fei-Fei Zhang, Er-Peng Song, Zeng-Mei Huang, Hui-Cong Luo, Fang-Jun Pan, Chang-Wang Tan, Feng
author_sort	Hu, Xin
title	In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)
title_short	In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)
title_full	In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)
title_fullStr	In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)
title_full_unstemmed	In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)
title_sort	in vitro study of the effects of angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (bbb)
description	It has been hypothesized that blood-brain barrier (BBB) dysfunction in Angiostrongylus cantonensis infection might be due to the apoptosis of the hosts' BBB cells. Here, we evaluated this hypothesis through several methods, all based on an in vitro mouse BBB model consisting of primary culture brain microvascular endothelial cells (BMECs) and brain astrocytic cells (BACs). In the present study, a four-hour percolation and HRP permeability experiment showed that A. cantonensis larvae extracts can increase the permeability of the BBB. Apoptosis among BMECs and BACs after exposure to larvae extracts was monitored by TUNEL and annexin-V-FITC/PI double staining. A. cantonensis larvae extracts were found to induce apoptosis in both BMECs and BACs. For this reason, we concluded that the induction of apoptosis might participate in the BBB dysfunction observed during angiostrongyliasis. Improved fundamental understanding of how A. cantonensis induces apoptosis may lead to new approaches to the treatment or prevention of this parasitic disease.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290544/
_version_	1611509409074118656

In Vitro Study of the Effects of Angiostrongylus cantonensis Larvae Extracts on Apoptosis and Dysfunction in the Blood-Brain Barrier (BBB)

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