Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2
The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infec...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
The Korean Society for Parasitology
2011
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279685/ |
| id |
pubmed-3279685 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-32796852012-02-21 Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2 Liao, Shunyao Liu, Yunqiang Zheng, Bing Cho, Pyo Yun Song, Hyun Ok Lee, Yun-Seok Jung, Suk-Yul Park, Hyun Brief Communication The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1α is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1α in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1α expressed by a lentiviral vector (LV HNF-1α) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1α was observed to influence promoter activity, suggesting that host HNF-1α interacts with the Sub2 gene. The Korean Society for Parasitology 2011-12 2011-12-16 /pmc/articles/PMC3279685/ /pubmed/22355214 http://dx.doi.org/10.3347/kjp.2011.49.4.431 Text en © 2011, Korean Society for Parasitology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Liao, Shunyao Liu, Yunqiang Zheng, Bing Cho, Pyo Yun Song, Hyun Ok Lee, Yun-Seok Jung, Suk-Yul Park, Hyun |
| spellingShingle |
Liao, Shunyao Liu, Yunqiang Zheng, Bing Cho, Pyo Yun Song, Hyun Ok Lee, Yun-Seok Jung, Suk-Yul Park, Hyun Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2 |
| author_facet |
Liao, Shunyao Liu, Yunqiang Zheng, Bing Cho, Pyo Yun Song, Hyun Ok Lee, Yun-Seok Jung, Suk-Yul Park, Hyun |
| author_sort |
Liao, Shunyao |
| title |
Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2 |
| title_short |
Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2 |
| title_full |
Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2 |
| title_fullStr |
Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2 |
| title_full_unstemmed |
Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2 |
| title_sort |
expression of exogenous human hepatic nuclear factor-1α by a lentiviral vector and its interactions with plasmodium falciparum subtilisin-like protease 2 |
| description |
The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1α is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1α in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1α expressed by a lentiviral vector (LV HNF-1α) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1α was observed to influence promoter activity, suggesting that host HNF-1α interacts with the Sub2 gene. |
| publisher |
The Korean Society for Parasitology |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279685/ |
| _version_ |
1611506111774457856 |