Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats

Objective: To evaluate the therapeutic effect of transplanted glial cell derived neurotrophic factor (GDNF) modified marrow stromal cells (MSCs) on an experimental ischemic brain injury based on the behavioral, morphological, and immunohistochemical observations. Methods: The MSCs from four-week new...

Full description

Bibliographic Details
Main Authors:	Wang, Yunliang, Geng, Tongchao, Ni, Amanda, Yin, Honglei, Han, Bing
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277278/

id	pubmed-3277278
recordtype	oai_dc
spelling	pubmed-32772782012-02-23 Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats Wang, Yunliang Geng, Tongchao Ni, Amanda Yin, Honglei Han, Bing Neuroscience Objective: To evaluate the therapeutic effect of transplanted glial cell derived neurotrophic factor (GDNF) modified marrow stromal cells (MSCs) on an experimental ischemic brain injury based on the behavioral, morphological, and immunohistochemical observations. Methods: The MSCs from four-week newborn rats were cultured in vitro. The cerebral ischemia and reperfusion model was established in adult Sprague–Dawley (SD) rats by using the suture method. Three days after model establishment, the animals were injected with prepared MSCs via their caudal veins. The animals were then divided into a sham-operation group, ischemia group, MSCs transplantation group, or GDNF+ MSCs transplantation group and were scored for their neurobehavioral manifestations at 3, 14, and 28 days after the transplantation was performed. At this time, the survival condition of intracerebral transplanted cells was measured by laser confocal microscopy while the effect of transplantation on the Generic Digital Beam Former (GDNF) expression in the ischemic brain tissue was evaluated. Results: The MSCs cells transfected with GDNF gene were characterized by green fluorescence. Three days after the transplantation, the animals that underwent the cell transplantation showed significantly better behavioral data than the controls. Fourteen days after transplantation, the animals transplanted with GDNF gene modified MSCs were better than those transplanted with common MSCs. As compared with common MSCs transplantation, GDNF+MSCs transplantation was significantly more effective in reducing apoptotic cell volume and enhancing Bcl-2 expression, which was favorable for the ischemic brain injury. Conclusions: Transplanted GDNF modified MSCs can improve the nervous function and have a protective effect on the ischemic brain injury through reducing apoptotic cell volume and enhancing the expression of anti-apoptotic gene Bcl-2. Frontiers Media S.A. 2011-12-22 /pmc/articles/PMC3277278/ /pubmed/22363270 http://dx.doi.org/10.3389/fnint.2011.00089 Text en Copyright © 2011 Wang, Geng, Ni, Yin and Han. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wang, Yunliang Geng, Tongchao Ni, Amanda Yin, Honglei Han, Bing
spellingShingle	Wang, Yunliang Geng, Tongchao Ni, Amanda Yin, Honglei Han, Bing Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats
author_facet	Wang, Yunliang Geng, Tongchao Ni, Amanda Yin, Honglei Han, Bing
author_sort	Wang, Yunliang
title	Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats
title_short	Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats
title_full	Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats
title_fullStr	Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats
title_full_unstemmed	Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats
title_sort	effects of transplanted gdnf gene modified marrow stromal cells on focal cerebral ischemia in rats
description	Objective: To evaluate the therapeutic effect of transplanted glial cell derived neurotrophic factor (GDNF) modified marrow stromal cells (MSCs) on an experimental ischemic brain injury based on the behavioral, morphological, and immunohistochemical observations. Methods: The MSCs from four-week newborn rats were cultured in vitro. The cerebral ischemia and reperfusion model was established in adult Sprague–Dawley (SD) rats by using the suture method. Three days after model establishment, the animals were injected with prepared MSCs via their caudal veins. The animals were then divided into a sham-operation group, ischemia group, MSCs transplantation group, or GDNF+ MSCs transplantation group and were scored for their neurobehavioral manifestations at 3, 14, and 28 days after the transplantation was performed. At this time, the survival condition of intracerebral transplanted cells was measured by laser confocal microscopy while the effect of transplantation on the Generic Digital Beam Former (GDNF) expression in the ischemic brain tissue was evaluated. Results: The MSCs cells transfected with GDNF gene were characterized by green fluorescence. Three days after the transplantation, the animals that underwent the cell transplantation showed significantly better behavioral data than the controls. Fourteen days after transplantation, the animals transplanted with GDNF gene modified MSCs were better than those transplanted with common MSCs. As compared with common MSCs transplantation, GDNF+MSCs transplantation was significantly more effective in reducing apoptotic cell volume and enhancing Bcl-2 expression, which was favorable for the ischemic brain injury. Conclusions: Transplanted GDNF modified MSCs can improve the nervous function and have a protective effect on the ischemic brain injury through reducing apoptotic cell volume and enhancing the expression of anti-apoptotic gene Bcl-2.
publisher	Frontiers Media S.A.
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277278/
_version_	1611505474529656832

Effects of transplanted GDNF gene modified marrow stromal cells on focal cerebral ischemia in rats

Similar Items