Luminescent detection of DNA-binding proteins

Transcription factors play a central role in cell development, differentiation and growth in biological systems due to their ability to regulate gene expression by binding to specific DNA sequences within the nucleus. The dysregulation of transcription factor signaling has been implicated in the pat...

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Main Authors:	Leung, Chung-Hang, Chan, Daniel Shiu-Hin, He, Hong-Zhang, Cheng, Zhen, Yang, Hui, Ma, Dik-Lung
Format:	Online
Language:	English
Published:	Oxford University Press 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273792/

id	pubmed-3273792
recordtype	oai_dc
spelling	pubmed-32737922012-02-07 Luminescent detection of DNA-binding proteins Leung, Chung-Hang Chan, Daniel Shiu-Hin He, Hong-Zhang Cheng, Zhen Yang, Hui Ma, Dik-Lung Survey and Summary Transcription factors play a central role in cell development, differentiation and growth in biological systems due to their ability to regulate gene expression by binding to specific DNA sequences within the nucleus. The dysregulation of transcription factor signaling has been implicated in the pathogenesis of a number of cancers, developmental disorders, inflammation and autoimmunity. There is thus a high demand for convenient high-throughput methodologies able to detect sequence-specific DNA-binding proteins and monitor their DNA-binding activities. Traditional approaches for protein detection include gel mobility shift assays, DNA footprinting and enzyme-linked immunosorbent assays (ELISAs) which tend to be tedious, time-consuming, and may necessitate the use of radiographic labeling. By contrast, luminescence technologies offer the potential for rapid, sensitive and low-cost detection that are amenable to high-throughput and real-time analysis. The discoveries of molecular beacons and aptamers have spearheaded the development of new luminescent methodologies for the detection of proteins over the last decade. We survey here recent advances in the development of luminescent detection methods for DNA-binding proteins, including those based on molecular beacons, aptamer beacons, label-free techniques and exonuclease protection. Oxford University Press 2012-02 2011-10-03 /pmc/articles/PMC3273792/ /pubmed/21967849 http://dx.doi.org/10.1093/nar/gkr763 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Leung, Chung-Hang Chan, Daniel Shiu-Hin He, Hong-Zhang Cheng, Zhen Yang, Hui Ma, Dik-Lung
spellingShingle	Leung, Chung-Hang Chan, Daniel Shiu-Hin He, Hong-Zhang Cheng, Zhen Yang, Hui Ma, Dik-Lung Luminescent detection of DNA-binding proteins
author_facet	Leung, Chung-Hang Chan, Daniel Shiu-Hin He, Hong-Zhang Cheng, Zhen Yang, Hui Ma, Dik-Lung
author_sort	Leung, Chung-Hang
title	Luminescent detection of DNA-binding proteins
title_short	Luminescent detection of DNA-binding proteins
title_full	Luminescent detection of DNA-binding proteins
title_fullStr	Luminescent detection of DNA-binding proteins
title_full_unstemmed	Luminescent detection of DNA-binding proteins
title_sort	luminescent detection of dna-binding proteins
description	Transcription factors play a central role in cell development, differentiation and growth in biological systems due to their ability to regulate gene expression by binding to specific DNA sequences within the nucleus. The dysregulation of transcription factor signaling has been implicated in the pathogenesis of a number of cancers, developmental disorders, inflammation and autoimmunity. There is thus a high demand for convenient high-throughput methodologies able to detect sequence-specific DNA-binding proteins and monitor their DNA-binding activities. Traditional approaches for protein detection include gel mobility shift assays, DNA footprinting and enzyme-linked immunosorbent assays (ELISAs) which tend to be tedious, time-consuming, and may necessitate the use of radiographic labeling. By contrast, luminescence technologies offer the potential for rapid, sensitive and low-cost detection that are amenable to high-throughput and real-time analysis. The discoveries of molecular beacons and aptamers have spearheaded the development of new luminescent methodologies for the detection of proteins over the last decade. We survey here recent advances in the development of luminescent detection methods for DNA-binding proteins, including those based on molecular beacons, aptamer beacons, label-free techniques and exonuclease protection.
publisher	Oxford University Press
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273792/
_version_	1611504203419615232

Luminescent detection of DNA-binding proteins

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