Endothelial and perivascular cells maintain haematopoietic stem cells

Endothelial and perivascular cells maintain haematopoietic stem cells

Multiple cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources o...

Full description

Bibliographic Details
Main Authors: Ding, Lei, Saunders, Thomas L., Enikolopov, Grigori, Morrison, Sean J.
Format: Online
Language:English
Published: 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270376/
id pubmed-3270376
recordtype oai_dc
spelling pubmed-32703762012-07-26 Endothelial and perivascular cells maintain haematopoietic stem cells Ding, Lei Saunders, Thomas L. Enikolopov, Grigori Morrison, Sean J. Article Multiple cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem Cell Factor (SCF) is a key niche component that maintains HSCs. Using Scfgfp knock-in mice we found Scf was primarily expressed by perivascular cells throughout bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, Nestin-Cre, or Nestin-CreER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or Leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance. 2012-01-25 /pmc/articles/PMC3270376/ /pubmed/22281595 http://dx.doi.org/10.1038/nature10783 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Ding, Lei
Saunders, Thomas L.
Enikolopov, Grigori
Morrison, Sean J.
spellingShingle Ding, Lei
Saunders, Thomas L.
Enikolopov, Grigori
Morrison, Sean J.
Endothelial and perivascular cells maintain haematopoietic stem cells
author_facet Ding, Lei
Saunders, Thomas L.
Enikolopov, Grigori
Morrison, Sean J.
author_sort Ding, Lei
title Endothelial and perivascular cells maintain haematopoietic stem cells
title_short Endothelial and perivascular cells maintain haematopoietic stem cells
title_full Endothelial and perivascular cells maintain haematopoietic stem cells
title_fullStr Endothelial and perivascular cells maintain haematopoietic stem cells
title_full_unstemmed Endothelial and perivascular cells maintain haematopoietic stem cells
title_sort endothelial and perivascular cells maintain haematopoietic stem cells
description Multiple cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem Cell Factor (SCF) is a key niche component that maintains HSCs. Using Scfgfp knock-in mice we found Scf was primarily expressed by perivascular cells throughout bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, Nestin-Cre, or Nestin-CreER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or Leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance.
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270376/
_version_ 1611503222426435584

Similar Items