Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation

Studies have compared the role of bone marrow aspirate cytology and trephine biopsy for diagnosing various hematological disorders but fewer studies have compared the relative value of imprint cytology with aspirate and trephine biopsy. The present study was conducted to compare the role of bone mar...

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Main Authors:	Chandra, Smita, Chandra, Harish
Format:	Online
Language:	English
Published:	PAGEPress Publications 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269795/

id	pubmed-3269795
recordtype	oai_dc
spelling	pubmed-32697952012-05-16 Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation Chandra, Smita Chandra, Harish Article Studies have compared the role of bone marrow aspirate cytology and trephine biopsy for diagnosing various hematological disorders but fewer studies have compared the relative value of imprint cytology with aspirate and trephine biopsy. The present study was conducted to compare the role of bone marrow aspirate, touch imprint and trephine biopsy to formulate an effective and rapid method for diagnosing wide spectrum of hematological diseases. The study included total 565 cases of bone marrow examination from January 2006 till May 2010. All the smears and sections were reviewed for morphological details and findings on aspirate, imprint and biopsy were compared to each other. The diagnostic accuracy of bone marrow aspirate was 77.5%, imprint cytology 83.7% and that of biopsy was of 99.2%. The study showed 78% positive correlation between aspirate and biopsy and 84.3% between imprint and biopsy; 93.3% cases of metastatic solid tumors were correctly diagnosed on imprint while only 70% cases were diagnosed on aspirate cytology. The study concludes that all the three preparations of aspirate, imprint and biopsy complement each other. The assessment of iron status by Perl's stain is most suitable on aspirate smears but trephine biopsy remains the gold standard for diagnosing granulomatous inflammation and hypoplastic/ aplastic anemia. Meticulously prepared imprint smears not only provide cellular composition of marrow but may also be helpful in defining the architecture of marrow especially in cases of metastatic solid tumors. Imprint cytology smears should be standard practice for evaluating any marrow. PAGEPress Publications 2011-10-21 /pmc/articles/PMC3269795/ /pubmed/22593814 http://dx.doi.org/10.4081/hr.2011.e22 Text en ©Copyright S. Chandra and H. Chandra, 2011 This work is licensed under a Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0). Licensee PAGEPress, Italy
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chandra, Smita Chandra, Harish
spellingShingle	Chandra, Smita Chandra, Harish Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation
author_facet	Chandra, Smita Chandra, Harish
author_sort	Chandra, Smita
title	Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation
title_short	Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation
title_full	Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation
title_fullStr	Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation
title_full_unstemmed	Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation
title_sort	comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation
description	Studies have compared the role of bone marrow aspirate cytology and trephine biopsy for diagnosing various hematological disorders but fewer studies have compared the relative value of imprint cytology with aspirate and trephine biopsy. The present study was conducted to compare the role of bone marrow aspirate, touch imprint and trephine biopsy to formulate an effective and rapid method for diagnosing wide spectrum of hematological diseases. The study included total 565 cases of bone marrow examination from January 2006 till May 2010. All the smears and sections were reviewed for morphological details and findings on aspirate, imprint and biopsy were compared to each other. The diagnostic accuracy of bone marrow aspirate was 77.5%, imprint cytology 83.7% and that of biopsy was of 99.2%. The study showed 78% positive correlation between aspirate and biopsy and 84.3% between imprint and biopsy; 93.3% cases of metastatic solid tumors were correctly diagnosed on imprint while only 70% cases were diagnosed on aspirate cytology. The study concludes that all the three preparations of aspirate, imprint and biopsy complement each other. The assessment of iron status by Perl's stain is most suitable on aspirate smears but trephine biopsy remains the gold standard for diagnosing granulomatous inflammation and hypoplastic/ aplastic anemia. Meticulously prepared imprint smears not only provide cellular composition of marrow but may also be helpful in defining the architecture of marrow especially in cases of metastatic solid tumors. Imprint cytology smears should be standard practice for evaluating any marrow.
publisher	PAGEPress Publications
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269795/
_version_	1611503083178688512

Comparison of bone marrow aspirate cytology, touch imprint cytology and trephine biopsy for bone marrow evaluation

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