Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp

The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown....

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Main Authors:	Yan, Xuechun, Ding, Lei, Li, Yunchao, Zhang, Xiaofeng, Liang, Yang, Sun, Xiaowen, Teng, Chun-Bo
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267759/

id	pubmed-3267759
recordtype	oai_dc
spelling	pubmed-32677592012-02-02 Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp Yan, Xuechun Ding, Lei Li, Yunchao Zhang, Xiaofeng Liang, Yang Sun, Xiaowen Teng, Chun-Bo Research Article The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown. It has been shown that a class of non-coding ∼22 nucleotide RNAs called microRNAs (miRNAs) play important roles in vertebrate development. They regulate gene expression through sequence-specific interactions with the 3′ untranslated regions (UTRs) of target mRNAs and thereby cause translational repression or mRNA destabilization. Intriguingly, the role of miRNAs in the skeletal muscle development of the common carp remains unknown. In this study, a small-RNA cDNA library was constructed from the skeletal muscle of the common carp, and Solexa sequencing technology was used to perform high throughput sequencing of the library. Subsequent bioinformatics analysis identified 188 conserved miRNAs and 7 novel miRNAs in the carp skeletal muscle. The miRNA expression profiling showed that, miR-1, miR-133a-3p, and miR-206 were specifically expressed in muscle-containing organs, and that miR-1, miR-21, miR-26a, miR-27a, miR-133a-3p, miR-206, miR-214 and miR-222 were differentially expressed in the process of skeletal muscle development of the common carp. This study provides a first identification and profiling of miRNAs related to the muscle biology of the common carp. Their identification could provide clues leading towards a better understanding of the molecular mechanisms of carp skeletal muscle development. Public Library of Science 2012-01-27 /pmc/articles/PMC3267759/ /pubmed/22303472 http://dx.doi.org/10.1371/journal.pone.0030925 Text en Yan et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yan, Xuechun Ding, Lei Li, Yunchao Zhang, Xiaofeng Liang, Yang Sun, Xiaowen Teng, Chun-Bo
spellingShingle	Yan, Xuechun Ding, Lei Li, Yunchao Zhang, Xiaofeng Liang, Yang Sun, Xiaowen Teng, Chun-Bo Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp
author_facet	Yan, Xuechun Ding, Lei Li, Yunchao Zhang, Xiaofeng Liang, Yang Sun, Xiaowen Teng, Chun-Bo
author_sort	Yan, Xuechun
title	Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp
title_short	Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp
title_full	Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp
title_fullStr	Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp
title_full_unstemmed	Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp
title_sort	identification and profiling of micrornas from skeletal muscle of the common carp
description	The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown. It has been shown that a class of non-coding ∼22 nucleotide RNAs called microRNAs (miRNAs) play important roles in vertebrate development. They regulate gene expression through sequence-specific interactions with the 3′ untranslated regions (UTRs) of target mRNAs and thereby cause translational repression or mRNA destabilization. Intriguingly, the role of miRNAs in the skeletal muscle development of the common carp remains unknown. In this study, a small-RNA cDNA library was constructed from the skeletal muscle of the common carp, and Solexa sequencing technology was used to perform high throughput sequencing of the library. Subsequent bioinformatics analysis identified 188 conserved miRNAs and 7 novel miRNAs in the carp skeletal muscle. The miRNA expression profiling showed that, miR-1, miR-133a-3p, and miR-206 were specifically expressed in muscle-containing organs, and that miR-1, miR-21, miR-26a, miR-27a, miR-133a-3p, miR-206, miR-214 and miR-222 were differentially expressed in the process of skeletal muscle development of the common carp. This study provides a first identification and profiling of miRNAs related to the muscle biology of the common carp. Their identification could provide clues leading towards a better understanding of the molecular mechanisms of carp skeletal muscle development.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267759/
_version_	1611502616268767232

Identification and Profiling of MicroRNAs from Skeletal Muscle of the Common Carp

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