Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution
Cell migration is crucial for both physiological and pathological processes. Current in vitro cell motility assays suffer from various drawbacks, including insufficient temporal and/or optical resolution, or the failure to include a controlled chemotactic stimulus. Here, we address these limitations...
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| Format: | Online |
| Language: | English |
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Public Library of Science
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261140/ |
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pubmed-3261140 |
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pubmed-32611402012-01-25 Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution Tong, ZiQiu Balzer, Eric M. Dallas, Matthew R. Hung, Wei-Chien Stebe, Kathleen J. Konstantopoulos, Konstantinos Research Article Cell migration is crucial for both physiological and pathological processes. Current in vitro cell motility assays suffer from various drawbacks, including insufficient temporal and/or optical resolution, or the failure to include a controlled chemotactic stimulus. Here, we address these limitations with a migration chamber that utilizes a self-sustaining chemotactic gradient to induce locomotion through confined environments that emulate physiological settings. Dynamic real-time analysis of both population-scale and single-cell movement are achieved at high resolution. Interior surfaces can be functionalized through adsorption of extracellular matrix components, and pharmacological agents can be administered to cells directly, or indirectly through the chemotactic reservoir. Direct comparison of multiple cell types can be achieved in a single enclosed system to compare inherent migratory potentials. Our novel microfluidic design is therefore a powerful tool for the study of cellular chemotaxis, and is suitable for a wide range of biological and biomedical applications. Public Library of Science 2012-01-18 /pmc/articles/PMC3261140/ /pubmed/22279529 http://dx.doi.org/10.1371/journal.pone.0029211 Text en Tong et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Tong, ZiQiu Balzer, Eric M. Dallas, Matthew R. Hung, Wei-Chien Stebe, Kathleen J. Konstantopoulos, Konstantinos |
| spellingShingle |
Tong, ZiQiu Balzer, Eric M. Dallas, Matthew R. Hung, Wei-Chien Stebe, Kathleen J. Konstantopoulos, Konstantinos Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution |
| author_facet |
Tong, ZiQiu Balzer, Eric M. Dallas, Matthew R. Hung, Wei-Chien Stebe, Kathleen J. Konstantopoulos, Konstantinos |
| author_sort |
Tong, ZiQiu |
| title |
Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution |
| title_short |
Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution |
| title_full |
Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution |
| title_fullStr |
Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution |
| title_full_unstemmed |
Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution |
| title_sort |
chemotaxis of cell populations through confined spaces at single-cell resolution |
| description |
Cell migration is crucial for both physiological and pathological processes. Current in vitro cell motility assays suffer from various drawbacks, including insufficient temporal and/or optical resolution, or the failure to include a controlled chemotactic stimulus. Here, we address these limitations with a migration chamber that utilizes a self-sustaining chemotactic gradient to induce locomotion through confined environments that emulate physiological settings. Dynamic real-time analysis of both population-scale and single-cell movement are achieved at high resolution. Interior surfaces can be functionalized through adsorption of extracellular matrix components, and pharmacological agents can be administered to cells directly, or indirectly through the chemotactic reservoir. Direct comparison of multiple cell types can be achieved in a single enclosed system to compare inherent migratory potentials. Our novel microfluidic design is therefore a powerful tool for the study of cellular chemotaxis, and is suitable for a wide range of biological and biomedical applications. |
| publisher |
Public Library of Science |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261140/ |
| _version_ |
1611500694010855424 |