Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate

The capacity of the rumen microbiota to produce volatile fatty acids (VFAs) has important implications in animal well-being and production. We investigated temporal changes of the rumen microbiota in response to butyrate infusion using pyrosequencing of the 16S rRNA gene. Twenty one phyla were ident...

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Main Authors:	Li, Robert W., Wu, Sitao, Baldwin, Ransom L., Li, Weizhong, Li, Congjun
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257242/

id	pubmed-3257242
recordtype	oai_dc
spelling	pubmed-32572422012-01-17 Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun Research Article The capacity of the rumen microbiota to produce volatile fatty acids (VFAs) has important implications in animal well-being and production. We investigated temporal changes of the rumen microbiota in response to butyrate infusion using pyrosequencing of the 16S rRNA gene. Twenty one phyla were identified in the rumen microbiota of dairy cows. The rumen microbiota harbored 54.5±6.1 genera (mean ± SD) and 127.3±4.4 operational taxonomic units (OTUs), respectively. However, the core microbiome comprised of 26 genera and 82 OTUs. Butyrate infusion altered molar percentages of 3 major VFAs. Butyrate perturbation had a profound impact on the rumen microbial composition. A 72 h-infusion led to a significant change in the numbers of sequence reads derived from 4 phyla, including 2 most abundant phyla, Bacteroidetes and Firmicutes. As many as 19 genera and 43 OTUs were significantly impacted by butyrate infusion. Elevated butyrate levels in the rumen seemingly had a stimulating effect on butyrate-producing bacteria populations. The resilience of the rumen microbial ecosystem was evident as the abundance of the microorganisms returned to their pre-disturbed status after infusion withdrawal. Our findings provide insight into perturbation dynamics of the rumen microbial ecosystem and should guide efforts in formulating optimal uses of probiotic bacteria treating human diseases. Public Library of Science 2012-01-12 /pmc/articles/PMC3257242/ /pubmed/22253719 http://dx.doi.org/10.1371/journal.pone.0029392 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun
spellingShingle	Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate
author_facet	Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun
author_sort	Li, Robert W.
title	Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate
title_short	Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate
title_full	Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate
title_fullStr	Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate
title_full_unstemmed	Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate
title_sort	perturbation dynamics of the rumen microbiota in response to exogenous butyrate
description	The capacity of the rumen microbiota to produce volatile fatty acids (VFAs) has important implications in animal well-being and production. We investigated temporal changes of the rumen microbiota in response to butyrate infusion using pyrosequencing of the 16S rRNA gene. Twenty one phyla were identified in the rumen microbiota of dairy cows. The rumen microbiota harbored 54.5±6.1 genera (mean ± SD) and 127.3±4.4 operational taxonomic units (OTUs), respectively. However, the core microbiome comprised of 26 genera and 82 OTUs. Butyrate infusion altered molar percentages of 3 major VFAs. Butyrate perturbation had a profound impact on the rumen microbial composition. A 72 h-infusion led to a significant change in the numbers of sequence reads derived from 4 phyla, including 2 most abundant phyla, Bacteroidetes and Firmicutes. As many as 19 genera and 43 OTUs were significantly impacted by butyrate infusion. Elevated butyrate levels in the rumen seemingly had a stimulating effect on butyrate-producing bacteria populations. The resilience of the rumen microbial ecosystem was evident as the abundance of the microorganisms returned to their pre-disturbed status after infusion withdrawal. Our findings provide insight into perturbation dynamics of the rumen microbial ecosystem and should guide efforts in formulating optimal uses of probiotic bacteria treating human diseases.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257242/
_version_	1611499614495571968

Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate

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