Comparison of three methods of DNA extraction from human bones with different degrees of degradation

There is a necessity for deceased identification as a result of many accidents and sometimes bones are the only accessible source of DNA. So far, a universal method that allows for extraction of DNA from materials at different stages of degradation does not exist. The aims of this study were: the co...

Full description

Bibliographic Details
Main Authors:	Jakubowska, Joanna, Maciejewska, Agnieszka, Pawłowski, Ryszard
Format:	Online
Language:	English
Published:	Springer-Verlag 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3254873/

id	pubmed-3254873
recordtype	oai_dc
spelling	pubmed-32548732012-01-20 Comparison of three methods of DNA extraction from human bones with different degrees of degradation Jakubowska, Joanna Maciejewska, Agnieszka Pawłowski, Ryszard Short Communication There is a necessity for deceased identification as a result of many accidents and sometimes bones are the only accessible source of DNA. So far, a universal method that allows for extraction of DNA from materials at different stages of degradation does not exist. The aims of this study were: the comparison of three methods of DNA extraction from bones with different degree of degradation and an evaluation of the usefulness of these methods in forensic genetics. The efficiency of DNA extraction, the degree of extract contamination by polymerase chain reaction (PCR) inhibitors and the possibility of determining the STR loci profile were especially being compared. Nuclear DNA from bones at different states of degradation was isolated using three methods: classical, organic phenol–chloroform extraction, DNA extraction from crystal aggregates and extraction by total demineralisation. Total demineralisation is the best method for most cases of DNA extraction from bones, although it does not provide pure DNA. DNA extraction from aggregates removes inhibitors much better and is also a good method of choice when identity determination of exhumed remains is necessary. In the case of not buried bones (remains found outside) total demineralisation or phenol–chloroform protocols are more efficient for successful DNA extraction. Springer-Verlag 2011-06-30 2012-01 /pmc/articles/PMC3254873/ /pubmed/21717154 http://dx.doi.org/10.1007/s00414-011-0590-5 Text en © The Author(s) 2011
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jakubowska, Joanna Maciejewska, Agnieszka Pawłowski, Ryszard
spellingShingle	Jakubowska, Joanna Maciejewska, Agnieszka Pawłowski, Ryszard Comparison of three methods of DNA extraction from human bones with different degrees of degradation
author_facet	Jakubowska, Joanna Maciejewska, Agnieszka Pawłowski, Ryszard
author_sort	Jakubowska, Joanna
title	Comparison of three methods of DNA extraction from human bones with different degrees of degradation
title_short	Comparison of three methods of DNA extraction from human bones with different degrees of degradation
title_full	Comparison of three methods of DNA extraction from human bones with different degrees of degradation
title_fullStr	Comparison of three methods of DNA extraction from human bones with different degrees of degradation
title_full_unstemmed	Comparison of three methods of DNA extraction from human bones with different degrees of degradation
title_sort	comparison of three methods of dna extraction from human bones with different degrees of degradation
description	There is a necessity for deceased identification as a result of many accidents and sometimes bones are the only accessible source of DNA. So far, a universal method that allows for extraction of DNA from materials at different stages of degradation does not exist. The aims of this study were: the comparison of three methods of DNA extraction from bones with different degree of degradation and an evaluation of the usefulness of these methods in forensic genetics. The efficiency of DNA extraction, the degree of extract contamination by polymerase chain reaction (PCR) inhibitors and the possibility of determining the STR loci profile were especially being compared. Nuclear DNA from bones at different states of degradation was isolated using three methods: classical, organic phenol–chloroform extraction, DNA extraction from crystal aggregates and extraction by total demineralisation. Total demineralisation is the best method for most cases of DNA extraction from bones, although it does not provide pure DNA. DNA extraction from aggregates removes inhibitors much better and is also a good method of choice when identity determination of exhumed remains is necessary. In the case of not buried bones (remains found outside) total demineralisation or phenol–chloroform protocols are more efficient for successful DNA extraction.
publisher	Springer-Verlag
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3254873/
_version_	1611499072478248960

Comparison of three methods of DNA extraction from human bones with different degrees of degradation

Similar Items