DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome

The DR region of particular primate species may display allelic polymorphism and gene copy number variation (region configuration polymorphism). The sum of these distinct types of polymorphism is defined as complexity. To date, however, the DR region of cynomolgus macaques (Macaca fascicularis) has...

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Main Authors:	Doxiadis, Gaby G. M., de Vos-Rouweler, Annemiek J. M., de Groot, Nanine, Otting, Nel, Bontrop, Ronald E.
Format:	Online
Language:	English
Published:	Springer-Verlag 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249155/

id	pubmed-3249155
recordtype	oai_dc
spelling	pubmed-32491552012-01-11 DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome Doxiadis, Gaby G. M. de Vos-Rouweler, Annemiek J. M. de Groot, Nanine Otting, Nel Bontrop, Ronald E. Original Paper The DR region of particular primate species may display allelic polymorphism and gene copy number variation (region configuration polymorphism). The sum of these distinct types of polymorphism is defined as complexity. To date, however, the DR region of cynomolgus macaques (Macaca fascicularis) has been poorly defined. Transcriptome analysis of a pedigreed colony, comprising animals from Indonesia and Indochina, revealed a total of 15 Mafa-DRA and 57 DRB alleles, specifying 28 different region configurations. The DRA alleles can be divided into two distinct lineages. One lineage is polymorphic, but the majority of the amino acid replacements map to the leader peptide. The second lineage is at best oligomorphic, and segregates with one specific Mafa-DRB allele. The number of Mafa-DRB genes ranges from two to five per haplotype. Due to the presence of pseudogenes, however, each haplotype encodes only one to three bona fide DRB transcripts. Depending on the region configuration in which the Mafa-DRB gene is embedded, identical alleles may display differential transcription levels. Region configurations appear to have been generated by recombination-like events. When genes or gene segments are relocated, it seems plausible that they may be placed in the context of distinct transcription control elements. As such, DRB region-related transcription level differences may add an extra layer of polymorphism to this section of the adaptive immune system. Springer-Verlag 2011-07-30 2012-01 /pmc/articles/PMC3249155/ /pubmed/21805219 http://dx.doi.org/10.1007/s00251-011-0561-5 Text en © The Author(s) 2011
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Doxiadis, Gaby G. M. de Vos-Rouweler, Annemiek J. M. de Groot, Nanine Otting, Nel Bontrop, Ronald E.
spellingShingle	Doxiadis, Gaby G. M. de Vos-Rouweler, Annemiek J. M. de Groot, Nanine Otting, Nel Bontrop, Ronald E. DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome
author_facet	Doxiadis, Gaby G. M. de Vos-Rouweler, Annemiek J. M. de Groot, Nanine Otting, Nel Bontrop, Ronald E.
author_sort	Doxiadis, Gaby G. M.
title	DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome
title_short	DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome
title_full	DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome
title_fullStr	DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome
title_full_unstemmed	DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome
title_sort	dr haplotype diversity of the cynomolgus macaque as defined by its transcriptome
description	The DR region of particular primate species may display allelic polymorphism and gene copy number variation (region configuration polymorphism). The sum of these distinct types of polymorphism is defined as complexity. To date, however, the DR region of cynomolgus macaques (Macaca fascicularis) has been poorly defined. Transcriptome analysis of a pedigreed colony, comprising animals from Indonesia and Indochina, revealed a total of 15 Mafa-DRA and 57 DRB alleles, specifying 28 different region configurations. The DRA alleles can be divided into two distinct lineages. One lineage is polymorphic, but the majority of the amino acid replacements map to the leader peptide. The second lineage is at best oligomorphic, and segregates with one specific Mafa-DRB allele. The number of Mafa-DRB genes ranges from two to five per haplotype. Due to the presence of pseudogenes, however, each haplotype encodes only one to three bona fide DRB transcripts. Depending on the region configuration in which the Mafa-DRB gene is embedded, identical alleles may display differential transcription levels. Region configurations appear to have been generated by recombination-like events. When genes or gene segments are relocated, it seems plausible that they may be placed in the context of distinct transcription control elements. As such, DRB region-related transcription level differences may add an extra layer of polymorphism to this section of the adaptive immune system.
publisher	Springer-Verlag
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249155/
_version_	1611497518178238464

DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome

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