An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation

Bacterial conjugation is a form of type IV secretion that transports protein and DNA to recipient cells. Specific bacteriophage exploit the conjugative pili and cell envelope spanning protein machinery of these systems to invade bacterial cells. Infection by phage R17 requires F-like pili and coupli...

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Main Authors:	Lang, Silvia, Kirchberger, Paul C, Gruber, Christian J, Redzej, Adam, Raffl, Sandra, Zellnig, Guenther, Zangger, Klaus, Zechner, Ellen L
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245843/

id	pubmed-3245843
recordtype	oai_dc
spelling	pubmed-32458432011-12-28 An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation Lang, Silvia Kirchberger, Paul C Gruber, Christian J Redzej, Adam Raffl, Sandra Zellnig, Guenther Zangger, Klaus Zechner, Ellen L Research Articles Bacterial conjugation is a form of type IV secretion that transports protein and DNA to recipient cells. Specific bacteriophage exploit the conjugative pili and cell envelope spanning protein machinery of these systems to invade bacterial cells. Infection by phage R17 requires F-like pili and coupling protein TraD, which gates the cytoplasmic entrance of the secretion channel. Here we investigate the role of TraD in R17 nucleoprotein uptake and find parallels to secretion mechanisms. The relaxosome of IncFII plasmid R1 is required. A ternary complex of plasmid oriT, TraD and a novel activation domain within the N-terminal 992 residues of TraI contributes a key mechanism involving relaxase-associated properties of TraI, protein interaction and the TraD ATPase. Helicase-associated activities of TraI are dispensable. These findings distinguish for the first time specific protein domains and complexes that process extracellular signals into distinct activation stages in the type IV initiation pathway. The study also provided insights into the evolutionary interplay of phage and the plasmids they exploit. Related plasmid F adapted to R17 independently of TraI. It follows that selection for phage resistance drives not only variation in TraA pilins but diversifies TraD and its binding partners in a plasmid-specific manner. Blackwell Publishing Ltd 2011-12 2011-11-08 /pmc/articles/PMC3245843/ /pubmed/22066957 http://dx.doi.org/10.1111/j.1365-2958.2011.07872.x Text en © 2011 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lang, Silvia Kirchberger, Paul C Gruber, Christian J Redzej, Adam Raffl, Sandra Zellnig, Guenther Zangger, Klaus Zechner, Ellen L
spellingShingle	Lang, Silvia Kirchberger, Paul C Gruber, Christian J Redzej, Adam Raffl, Sandra Zellnig, Guenther Zangger, Klaus Zechner, Ellen L An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation
author_facet	Lang, Silvia Kirchberger, Paul C Gruber, Christian J Redzej, Adam Raffl, Sandra Zellnig, Guenther Zangger, Klaus Zechner, Ellen L
author_sort	Lang, Silvia
title	An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation
title_short	An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation
title_full	An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation
title_fullStr	An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation
title_full_unstemmed	An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation
title_sort	activation domain of plasmid r1 trai protein delineates stages of gene transfer initiation
description	Bacterial conjugation is a form of type IV secretion that transports protein and DNA to recipient cells. Specific bacteriophage exploit the conjugative pili and cell envelope spanning protein machinery of these systems to invade bacterial cells. Infection by phage R17 requires F-like pili and coupling protein TraD, which gates the cytoplasmic entrance of the secretion channel. Here we investigate the role of TraD in R17 nucleoprotein uptake and find parallels to secretion mechanisms. The relaxosome of IncFII plasmid R1 is required. A ternary complex of plasmid oriT, TraD and a novel activation domain within the N-terminal 992 residues of TraI contributes a key mechanism involving relaxase-associated properties of TraI, protein interaction and the TraD ATPase. Helicase-associated activities of TraI are dispensable. These findings distinguish for the first time specific protein domains and complexes that process extracellular signals into distinct activation stages in the type IV initiation pathway. The study also provided insights into the evolutionary interplay of phage and the plasmids they exploit. Related plasmid F adapted to R17 independently of TraI. It follows that selection for phage resistance drives not only variation in TraA pilins but diversifies TraD and its binding partners in a plasmid-specific manner.
publisher	Blackwell Publishing Ltd
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245843/
_version_	1611496492226314240

An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation

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