Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea

Plasmodium vivax is highly endemic in the lowlands of Papua New Guinea and accounts for a large proportion of the malaria cases in children less than 5 years of age. We collected 2117 blood samples at 2-monthly intervals from a cohort of 268 children aged 1 to 4.5 years and estimated the diversity...

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Main Authors:	Koepfli, Cristian, Ross, Amanda, Kiniboro, Benson, Smith, Thomas A., Zimmerman, Peter A., Siba, Peter, Mueller, Ivo, Felger, Ingrid
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243695/

id	pubmed-3243695
recordtype	oai_dc
spelling	pubmed-32436952011-12-28 Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea Koepfli, Cristian Ross, Amanda Kiniboro, Benson Smith, Thomas A. Zimmerman, Peter A. Siba, Peter Mueller, Ivo Felger, Ingrid Research Article Plasmodium vivax is highly endemic in the lowlands of Papua New Guinea and accounts for a large proportion of the malaria cases in children less than 5 years of age. We collected 2117 blood samples at 2-monthly intervals from a cohort of 268 children aged 1 to 4.5 years and estimated the diversity and multiplicity of P. vivax infection. All P. vivax clones were genotyped using the merozoite surface protein 1 F3 fragment (msp1F3) and the microsatellite MS16 as molecular markers. High diversity was observed with msp1F3 (H E = 88.1%) and MS16 (H E = 97.8%). Of the 1162 P. vivax positive samples, 74% harbored multi-clone infections with a mean multiplicity of 2.7 (IQR = 1–3). The multiplicity of P. vivax infection increased slightly with age (P = 0.02), with the strongest increase in very young children. Intensified efforts to control malaria can benefit from knowledge of the diversity and MOI both for assessing the endemic situation and monitoring the effects of interventions. Public Library of Science 2011-12-20 /pmc/articles/PMC3243695/ /pubmed/22206027 http://dx.doi.org/10.1371/journal.pntd.0001424 Text en Koepfli et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Koepfli, Cristian Ross, Amanda Kiniboro, Benson Smith, Thomas A. Zimmerman, Peter A. Siba, Peter Mueller, Ivo Felger, Ingrid
spellingShingle	Koepfli, Cristian Ross, Amanda Kiniboro, Benson Smith, Thomas A. Zimmerman, Peter A. Siba, Peter Mueller, Ivo Felger, Ingrid Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea
author_facet	Koepfli, Cristian Ross, Amanda Kiniboro, Benson Smith, Thomas A. Zimmerman, Peter A. Siba, Peter Mueller, Ivo Felger, Ingrid
author_sort	Koepfli, Cristian
title	Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea
title_short	Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea
title_full	Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea
title_fullStr	Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea
title_full_unstemmed	Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea
title_sort	multiplicity and diversity of plasmodium vivax infections in a highly endemic region in papua new guinea
description	Plasmodium vivax is highly endemic in the lowlands of Papua New Guinea and accounts for a large proportion of the malaria cases in children less than 5 years of age. We collected 2117 blood samples at 2-monthly intervals from a cohort of 268 children aged 1 to 4.5 years and estimated the diversity and multiplicity of P. vivax infection. All P. vivax clones were genotyped using the merozoite surface protein 1 F3 fragment (msp1F3) and the microsatellite MS16 as molecular markers. High diversity was observed with msp1F3 (H E = 88.1%) and MS16 (H E = 97.8%). Of the 1162 P. vivax positive samples, 74% harbored multi-clone infections with a mean multiplicity of 2.7 (IQR = 1–3). The multiplicity of P. vivax infection increased slightly with age (P = 0.02), with the strongest increase in very young children. Intensified efforts to control malaria can benefit from knowledge of the diversity and MOI both for assessing the endemic situation and monitoring the effects of interventions.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243695/
_version_	1611495959026466816

Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea

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