Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin

Equol, an isoflavonoid metabolite produced from the dietary isoflavone daidzein by the gut microflora in mammals, has been found to protect not only against ultraviolet (UV) radiation-induced cutaneous inflammation and photoimmune suppression, but also have anti-photocarcinogenic properties in mice....

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Main Author:	Widyarini, Sitarina
Format:	Online
Language:	English
Published:	The Korean Society of Veterinary Science 2006
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242119/

id	pubmed-3242119
recordtype	oai_dc
spelling	pubmed-32421192011-12-22 Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin Widyarini, Sitarina Original Article Equol, an isoflavonoid metabolite produced from the dietary isoflavone daidzein by the gut microflora in mammals, has been found to protect not only against ultraviolet (UV) radiation-induced cutaneous inflammation and photoimmune suppression, but also have anti-photocarcinogenic properties in mice. Because the state of DNA damage has been correlated with suppression of the immune system and photocarcinogenesis, we have therefore examined the potential of equol to offer protection from solar-simulated UV (SSUV) radiation-induced DNA damage in hairless mice by the immunohistochemical approach using monoclonal antibody specific for cyclobutane pyrimidine dimers (CPDs; H3 antibody). Topical application of 20 µM equol lotion, which was applied both before and after SSUV significantly reduced the number of CPDs. This reduction was evident immediately after SSUV exposure, at 1 h after exposure, and at 24 h after exposure, revealing 54%, 50%, and 26% reduction in CPDs, respectively. When the same concentration was applied for 5 consecutive days after SSUV exposure, there was no significant difference in the reduction of CPDs immediately after SSUV irradiation or at 1 hour afterwards, but there were significant reductions of 23% and 42% at 24 and 48 h after SSUV exposure, respectively. Despite apparently reducing the number of CPDs post-SSUV, topically applied equol did not appear to increase the rate of dimer removal. To conclude, equol applied topically prior to SSUV irradiation offers protection against CPD formation in hairless mice, possibly by acting as a suncreen and thus inhibiting DNA photodamage. The Korean Society of Veterinary Science 2006-09 2006-09-30 /pmc/articles/PMC3242119/ /pubmed/16871014 http://dx.doi.org/10.4142/jvs.2006.7.3.217 Text en Copyright © 2006 The Korean Society of Veterinary Science
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Widyarini, Sitarina
spellingShingle	Widyarini, Sitarina Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin
author_facet	Widyarini, Sitarina
author_sort	Widyarini, Sitarina
title	Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin
title_short	Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin
title_full	Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin
title_fullStr	Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin
title_full_unstemmed	Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin
title_sort	protective effect of the isoflavone equol against dna damage induced by ultraviolet radiation to hairless mouse skin
description	Equol, an isoflavonoid metabolite produced from the dietary isoflavone daidzein by the gut microflora in mammals, has been found to protect not only against ultraviolet (UV) radiation-induced cutaneous inflammation and photoimmune suppression, but also have anti-photocarcinogenic properties in mice. Because the state of DNA damage has been correlated with suppression of the immune system and photocarcinogenesis, we have therefore examined the potential of equol to offer protection from solar-simulated UV (SSUV) radiation-induced DNA damage in hairless mice by the immunohistochemical approach using monoclonal antibody specific for cyclobutane pyrimidine dimers (CPDs; H3 antibody). Topical application of 20 µM equol lotion, which was applied both before and after SSUV significantly reduced the number of CPDs. This reduction was evident immediately after SSUV exposure, at 1 h after exposure, and at 24 h after exposure, revealing 54%, 50%, and 26% reduction in CPDs, respectively. When the same concentration was applied for 5 consecutive days after SSUV exposure, there was no significant difference in the reduction of CPDs immediately after SSUV irradiation or at 1 hour afterwards, but there were significant reductions of 23% and 42% at 24 and 48 h after SSUV exposure, respectively. Despite apparently reducing the number of CPDs post-SSUV, topically applied equol did not appear to increase the rate of dimer removal. To conclude, equol applied topically prior to SSUV irradiation offers protection against CPD formation in hairless mice, possibly by acting as a suncreen and thus inhibiting DNA photodamage.
publisher	The Korean Society of Veterinary Science
publishDate	2006
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242119/
_version_	1611495691869224960

Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin

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