Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm

For most protein coding genes, termination of transcription by RNA polymerase II is preceded by an endonucleolytic cleavage of the nascent transcript. The 3′ product of this cleavage is rapidly degraded via the 5′ exoribonuclease Rat1p which is thought to destabilize the RNA polymerase II complex. I...

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Main Authors:	Meaux, Stacie, Lavoie, Mathieu, Gagnon, Jules, Abou Elela, Sherif, van Hoof, Ambro
Format:	Online
Language:	English
Published:	Oxford University Press 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241649/

id	pubmed-3241649
recordtype	oai_dc
spelling	pubmed-32416492011-12-19 Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm Meaux, Stacie Lavoie, Mathieu Gagnon, Jules Abou Elela, Sherif van Hoof, Ambro RNA For most protein coding genes, termination of transcription by RNA polymerase II is preceded by an endonucleolytic cleavage of the nascent transcript. The 3′ product of this cleavage is rapidly degraded via the 5′ exoribonuclease Rat1p which is thought to destabilize the RNA polymerase II complex. It is not clear whether RNA cleavage is sufficient to trigger nuclear RNA degradation and transcription termination or whether the fate of the RNA depends on additional elements. For most mRNAs, this cleavage is mediated by the cleavage and polyadenylation machinery, but it can also be mediated by Rnt1p. We show that Rnt1p cleavage of an mRNA is not sufficient to trigger nuclear degradation or transcription termination. Insertion of an Rnt1p target site into a reporter mRNA did not block transcription downstream of the cleavage site, but instead produced two unstable cleavage products, neither of which were stabilized by inactivation of Rat1p. In contrast, the 3′ and 5′ cleavage products were stabilized by the deletion of the cytoplasmic 5′ exoribonuclease (Xrn1p) or by inactivation of the cytoplasmic RNA exosome. These data indicate that transcription termination and nuclear degradation is not the default fate of cleaved RNAs and that specific promoter and/or sequence elements are required to determine the fate of the cleavage products. Oxford University Press 2011-11 2011-08-05 /pmc/articles/PMC3241649/ /pubmed/21821655 http://dx.doi.org/10.1093/nar/gkr627 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Meaux, Stacie Lavoie, Mathieu Gagnon, Jules Abou Elela, Sherif van Hoof, Ambro
spellingShingle	Meaux, Stacie Lavoie, Mathieu Gagnon, Jules Abou Elela, Sherif van Hoof, Ambro Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm
author_facet	Meaux, Stacie Lavoie, Mathieu Gagnon, Jules Abou Elela, Sherif van Hoof, Ambro
author_sort	Meaux, Stacie
title	Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm
title_short	Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm
title_full	Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm
title_fullStr	Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm
title_full_unstemmed	Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm
title_sort	reporter mrnas cleaved by rnt1p are exported and degraded in the cytoplasm
description	For most protein coding genes, termination of transcription by RNA polymerase II is preceded by an endonucleolytic cleavage of the nascent transcript. The 3′ product of this cleavage is rapidly degraded via the 5′ exoribonuclease Rat1p which is thought to destabilize the RNA polymerase II complex. It is not clear whether RNA cleavage is sufficient to trigger nuclear RNA degradation and transcription termination or whether the fate of the RNA depends on additional elements. For most mRNAs, this cleavage is mediated by the cleavage and polyadenylation machinery, but it can also be mediated by Rnt1p. We show that Rnt1p cleavage of an mRNA is not sufficient to trigger nuclear degradation or transcription termination. Insertion of an Rnt1p target site into a reporter mRNA did not block transcription downstream of the cleavage site, but instead produced two unstable cleavage products, neither of which were stabilized by inactivation of Rat1p. In contrast, the 3′ and 5′ cleavage products were stabilized by the deletion of the cytoplasmic 5′ exoribonuclease (Xrn1p) or by inactivation of the cytoplasmic RNA exosome. These data indicate that transcription termination and nuclear degradation is not the default fate of cleaved RNAs and that specific promoter and/or sequence elements are required to determine the fate of the cleavage products.
publisher	Oxford University Press
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241649/
_version_	1611495585305591808

Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm

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