Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy

Fast three-(3D) imaging requires parallel optical slicing of a specimen with an efficient detection scheme. The generation of multiple localized dot-like excitation structures solves the problem of simultaneous slicing multiple specimen layers, but an efficient detection scheme is necessary. Confoca...

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Main Authors:	Mondal, Partha Pratim, Diaspro, Alberto
Format:	Online
Language:	English
Published:	Nature Publishing Group 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240976/

id	pubmed-3240976
recordtype	oai_dc
spelling	pubmed-32409762011-12-22 Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy Mondal, Partha Pratim Diaspro, Alberto Article Fast three-(3D) imaging requires parallel optical slicing of a specimen with an efficient detection scheme. The generation of multiple localized dot-like excitation structures solves the problem of simultaneous slicing multiple specimen layers, but an efficient detection scheme is necessary. Confocal theta detection (detection at 90° to the optical axis) provides a suitable detection platform that is capable of cross-talk-free fluorescence detection from each nanodot (axial dimension ≈ 150 nm). Additionally, this technique has the unique feature of imaging a specimen at a large working distance with super-resolution capabilities. Polarization studies show distinct field structures for fixed and fluid samples, indicating a non-negligible field-dipole interaction. The realization of the proposed imaging technique will advance and diversify multiphoton fluorescence microscopy for numerous applications in nanobioimaging and optical engineering. Nature Publishing Group 2011-11-09 /pmc/articles/PMC3240976/ /pubmed/22355665 http://dx.doi.org/10.1038/srep00149 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Mondal, Partha Pratim Diaspro, Alberto
spellingShingle	Mondal, Partha Pratim Diaspro, Alberto Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy
author_facet	Mondal, Partha Pratim Diaspro, Alberto
author_sort	Mondal, Partha Pratim
title	Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy
title_short	Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy
title_full	Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy
title_fullStr	Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy
title_full_unstemmed	Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy
title_sort	simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy
description	Fast three-(3D) imaging requires parallel optical slicing of a specimen with an efficient detection scheme. The generation of multiple localized dot-like excitation structures solves the problem of simultaneous slicing multiple specimen layers, but an efficient detection scheme is necessary. Confocal theta detection (detection at 90° to the optical axis) provides a suitable detection platform that is capable of cross-talk-free fluorescence detection from each nanodot (axial dimension ≈ 150 nm). Additionally, this technique has the unique feature of imaging a specimen at a large working distance with super-resolution capabilities. Polarization studies show distinct field structures for fixed and fluid samples, indicating a non-negligible field-dipole interaction. The realization of the proposed imaging technique will advance and diversify multiphoton fluorescence microscopy for numerous applications in nanobioimaging and optical engineering.
publisher	Nature Publishing Group
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240976/
_version_	1611495472058335232

Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy

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