Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases

Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases

Yellow catfish (Pelteobagrus fulvidraco) is one of the most important freshwater aquaculture species in China. However, its small size and lower meat yield limit its edible value. Myostatin (MSTN) is a negative regulator of mammalian muscle growth. But, the function of Mstn in fish remains elusive....

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Main Authors: Dong, Zhangji, Ge, Jiachun, Li, Kui, Xu, Zhiqiang, Liang, Dong, Li, Jingyun, Li, Junbo, Jia, Wenshuang, Li, Yuehua, Dong, Xiaohua, Cao, Shasha, Wang, Xiaoxiao, Pan, Jianlin, Zhao, Qingshun
Format: Online
Language:English
Published: Public Library of Science 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237566/
id pubmed-3237566
recordtype oai_dc
spelling pubmed-32375662011-12-22 Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases Dong, Zhangji Ge, Jiachun Li, Kui Xu, Zhiqiang Liang, Dong Li, Jingyun Li, Junbo Jia, Wenshuang Li, Yuehua Dong, Xiaohua Cao, Shasha Wang, Xiaoxiao Pan, Jianlin Zhao, Qingshun Research Article Yellow catfish (Pelteobagrus fulvidraco) is one of the most important freshwater aquaculture species in China. However, its small size and lower meat yield limit its edible value. Myostatin (MSTN) is a negative regulator of mammalian muscle growth. But, the function of Mstn in fish remains elusive. To explore roles of mstn gene in fish growth and create a strain of yellow catfish with high amount of muscle mass, we performed targeted disruption of mstn in yellow catfish using engineered zinc-finger nucleases (ZFNs). Employing zebrafish embryos as a screening system to identify ZFN activity, we obtained one pair of ZFNs that can edit mstn in yellow catfish genome. Using the ZFNs, we successfully obtained two founders (Founder July29-7 and Founder July29-8) carrying mutated mstn gene in their germ cells. The mutated mstn allele inherited from Founder July29-7 was a null allele (mstnnju6) containing a 4 bp insertion, predicted to encode function null Mstn. The mutated mstn inherited from Founder July29-8 was a complex type of mutation (mstnnju7), predicted to encode a protein lacking two amino acids in the N-terminal secretory signal of Mstn. Totally, we obtained 6 mstnnju6/+ and 14 mstnnju7/+ yellow catfish. To our best knowledge, this is the first endogenous gene knockout in aquaculture fish. Our result will help in understanding the roles of mstn gene in fish. Public Library of Science 2011-12-14 /pmc/articles/PMC3237566/ /pubmed/22194943 http://dx.doi.org/10.1371/journal.pone.0028897 Text en Dong et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Dong, Zhangji
Ge, Jiachun
Li, Kui
Xu, Zhiqiang
Liang, Dong
Li, Jingyun
Li, Junbo
Jia, Wenshuang
Li, Yuehua
Dong, Xiaohua
Cao, Shasha
Wang, Xiaoxiao
Pan, Jianlin
Zhao, Qingshun
spellingShingle Dong, Zhangji
Ge, Jiachun
Li, Kui
Xu, Zhiqiang
Liang, Dong
Li, Jingyun
Li, Junbo
Jia, Wenshuang
Li, Yuehua
Dong, Xiaohua
Cao, Shasha
Wang, Xiaoxiao
Pan, Jianlin
Zhao, Qingshun
Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases
author_facet Dong, Zhangji
Ge, Jiachun
Li, Kui
Xu, Zhiqiang
Liang, Dong
Li, Jingyun
Li, Junbo
Jia, Wenshuang
Li, Yuehua
Dong, Xiaohua
Cao, Shasha
Wang, Xiaoxiao
Pan, Jianlin
Zhao, Qingshun
author_sort Dong, Zhangji
title Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases
title_short Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases
title_full Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases
title_fullStr Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases
title_full_unstemmed Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases
title_sort heritable targeted inactivation of myostatin gene in yellow catfish (pelteobagrus fulvidraco) using engineered zinc finger nucleases
description Yellow catfish (Pelteobagrus fulvidraco) is one of the most important freshwater aquaculture species in China. However, its small size and lower meat yield limit its edible value. Myostatin (MSTN) is a negative regulator of mammalian muscle growth. But, the function of Mstn in fish remains elusive. To explore roles of mstn gene in fish growth and create a strain of yellow catfish with high amount of muscle mass, we performed targeted disruption of mstn in yellow catfish using engineered zinc-finger nucleases (ZFNs). Employing zebrafish embryos as a screening system to identify ZFN activity, we obtained one pair of ZFNs that can edit mstn in yellow catfish genome. Using the ZFNs, we successfully obtained two founders (Founder July29-7 and Founder July29-8) carrying mutated mstn gene in their germ cells. The mutated mstn allele inherited from Founder July29-7 was a null allele (mstnnju6) containing a 4 bp insertion, predicted to encode function null Mstn. The mutated mstn inherited from Founder July29-8 was a complex type of mutation (mstnnju7), predicted to encode a protein lacking two amino acids in the N-terminal secretory signal of Mstn. Totally, we obtained 6 mstnnju6/+ and 14 mstnnju7/+ yellow catfish. To our best knowledge, this is the first endogenous gene knockout in aquaculture fish. Our result will help in understanding the roles of mstn gene in fish.
publisher Public Library of Science
publishDate 2011
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237566/
_version_ 1611493968974970880

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