Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress

The cloning and characterization of a gene (MsHSP23) coding for a heat shock protein in alfalfa in a prokaryotic and model plant system is described. MsHSP23 contains a 633 bp ORF encoding a polypeptide of 213 amino acids and exhibits greater sequence similarity to mitochondrial sHSPs from dicotyled...

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Main Authors:	Lee, Ki-Won, Cha, Joon-Yung, Kim, Kyung-Hee, Kim, Yong-Goo, Lee, Byung-Hyun, Lee, Sang-Hoon
Format:	Online
Language:	English
Published:	Springer Netherlands 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235403/

id	pubmed-3235403
recordtype	oai_dc
spelling	pubmed-32354032011-12-27 Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress Lee, Ki-Won Cha, Joon-Yung Kim, Kyung-Hee Kim, Yong-Goo Lee, Byung-Hyun Lee, Sang-Hoon Original Research Paper The cloning and characterization of a gene (MsHSP23) coding for a heat shock protein in alfalfa in a prokaryotic and model plant system is described. MsHSP23 contains a 633 bp ORF encoding a polypeptide of 213 amino acids and exhibits greater sequence similarity to mitochondrial sHSPs from dicotyledons than to those from monocotyledons. When expressed in bacteria, recombinant MsHSP23 conferred tolerance to salinity and arsenic stress. Furthermore, MsHSP23 was cloned in a plant expressing vector and transformed into tobacco, a eukaryotic model organism. The transgenic plants exhibited enhanced tolerance to salinity and arsenic stress under ex vitro conditions. In comparison to wild type plants, the transgenic plants exhibited significantly lower electrolyte leakage. Moreover, the transgenic plants had superior germination rates when placed on medium containing arsenic. Taken together, these overexpression results imply that MsHSP23 plays an important role in salinity and arsenic stress tolerance in transgenic tobacco. This approach could be useful to develop stress tolerant crops including forage crops. Springer Netherlands 2011-11-30 2012-01 /pmc/articles/PMC3235403/ /pubmed/22127759 http://dx.doi.org/10.1007/s10529-011-0750-1 Text en © The Author(s) 2011
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lee, Ki-Won Cha, Joon-Yung Kim, Kyung-Hee Kim, Yong-Goo Lee, Byung-Hyun Lee, Sang-Hoon
spellingShingle	Lee, Ki-Won Cha, Joon-Yung Kim, Kyung-Hee Kim, Yong-Goo Lee, Byung-Hyun Lee, Sang-Hoon Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress
author_facet	Lee, Ki-Won Cha, Joon-Yung Kim, Kyung-Hee Kim, Yong-Goo Lee, Byung-Hyun Lee, Sang-Hoon
author_sort	Lee, Ki-Won
title	Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress
title_short	Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress
title_full	Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress
title_fullStr	Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress
title_full_unstemmed	Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress
title_sort	overexpression of alfalfa mitochondrial hsp23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress
description	The cloning and characterization of a gene (MsHSP23) coding for a heat shock protein in alfalfa in a prokaryotic and model plant system is described. MsHSP23 contains a 633 bp ORF encoding a polypeptide of 213 amino acids and exhibits greater sequence similarity to mitochondrial sHSPs from dicotyledons than to those from monocotyledons. When expressed in bacteria, recombinant MsHSP23 conferred tolerance to salinity and arsenic stress. Furthermore, MsHSP23 was cloned in a plant expressing vector and transformed into tobacco, a eukaryotic model organism. The transgenic plants exhibited enhanced tolerance to salinity and arsenic stress under ex vitro conditions. In comparison to wild type plants, the transgenic plants exhibited significantly lower electrolyte leakage. Moreover, the transgenic plants had superior germination rates when placed on medium containing arsenic. Taken together, these overexpression results imply that MsHSP23 plays an important role in salinity and arsenic stress tolerance in transgenic tobacco. This approach could be useful to develop stress tolerant crops including forage crops.
publisher	Springer Netherlands
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235403/
_version_	1611493138500681728

Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress

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