Taxonomy of Penicillium section Citrina

Species of Penicillium section Citrina have a worldwide distribution and occur commonly in soils. The section is here delimited using a combination of phenotypic characters and sequences of the nuclear ribosomal RNA gene operon, including the internal transcribed spacer regions ITS1 and ITS2, the 5....

Full description

Bibliographic Details
Main Authors:	Houbraken, J., Frisvad, J.C., Samson, R.A.
Format:	Online
Language:	English
Published:	CBS Fungal Biodiversity Centre 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233908/

id	pubmed-3233908
recordtype	oai_dc
spelling	pubmed-32339082012-02-03 Taxonomy of Penicillium section Citrina Houbraken, J. Frisvad, J.C. Samson, R.A. Articles Species of Penicillium section Citrina have a worldwide distribution and occur commonly in soils. The section is here delimited using a combination of phenotypic characters and sequences of the nuclear ribosomal RNA gene operon, including the internal transcribed spacer regions ITS1 and ITS2, the 5.8S nrDNA (ITS) and partial RPB2 sequences. Species assigned to section Citrina share the production of symmetrically biverticillate conidiophores, flask shaped phialides (7.0–9.0 μm long) and relatively small conidia (2.0–3.0 μm diam). Some species can produce greyish-brown coloured cleistothecia containing flanged ascospores. In the present study, more than 250 isolates presumably belonging to section Citrina were examined using a combined analysis of phenotypic and physiological characters, extrolite profiles and ITS, β-tubulin and/or calmodulin sequences. Section Citrina includes 39 species, and 17 of those are described here as new. The most important phenotypic characters for distinguishing species are growth rates and colony reverse colours on the agar media CYA, MEA and YES; shape, size and ornamentation of conidia and the production of sclerotia or cleistothecia. Temperature-growth profiles were made for all examined species and are a valuable character characters for species identification. Species centered around P. citrinum generally have a higher maximum growth temperature (33–36 °C) than species related to P. westlingii (27–33 °C). Extrolite patterns and partial calmodulin and β-tubulin sequences can be used for sequence based identification and resolved all species. In contrast, ITS sequences were less variable and only 55 % of the species could be unambiguously identified with this locus. CBS Fungal Biodiversity Centre 2011-11-15 /pmc/articles/PMC3233908/ /pubmed/22308046 http://dx.doi.org/10.3114/sim.2011.70.02 Text en Copyright 2011 CBS-KNAW Fungal Biodiversity Centre You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial: You may not use this work for commercial purposes. No derivative works: You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author's moral rights.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Houbraken, J. Frisvad, J.C. Samson, R.A.
spellingShingle	Houbraken, J. Frisvad, J.C. Samson, R.A. Taxonomy of Penicillium section Citrina
author_facet	Houbraken, J. Frisvad, J.C. Samson, R.A.
author_sort	Houbraken, J.
title	Taxonomy of Penicillium section Citrina
title_short	Taxonomy of Penicillium section Citrina
title_full	Taxonomy of Penicillium section Citrina
title_fullStr	Taxonomy of Penicillium section Citrina
title_full_unstemmed	Taxonomy of Penicillium section Citrina
title_sort	taxonomy of penicillium section citrina
description	Species of Penicillium section Citrina have a worldwide distribution and occur commonly in soils. The section is here delimited using a combination of phenotypic characters and sequences of the nuclear ribosomal RNA gene operon, including the internal transcribed spacer regions ITS1 and ITS2, the 5.8S nrDNA (ITS) and partial RPB2 sequences. Species assigned to section Citrina share the production of symmetrically biverticillate conidiophores, flask shaped phialides (7.0–9.0 μm long) and relatively small conidia (2.0–3.0 μm diam). Some species can produce greyish-brown coloured cleistothecia containing flanged ascospores. In the present study, more than 250 isolates presumably belonging to section Citrina were examined using a combined analysis of phenotypic and physiological characters, extrolite profiles and ITS, β-tubulin and/or calmodulin sequences. Section Citrina includes 39 species, and 17 of those are described here as new. The most important phenotypic characters for distinguishing species are growth rates and colony reverse colours on the agar media CYA, MEA and YES; shape, size and ornamentation of conidia and the production of sclerotia or cleistothecia. Temperature-growth profiles were made for all examined species and are a valuable character characters for species identification. Species centered around P. citrinum generally have a higher maximum growth temperature (33–36 °C) than species related to P. westlingii (27–33 °C). Extrolite patterns and partial calmodulin and β-tubulin sequences can be used for sequence based identification and resolved all species. In contrast, ITS sequences were less variable and only 55 % of the species could be unambiguously identified with this locus.
publisher	CBS Fungal Biodiversity Centre
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233908/
_version_	1611492784835919872

Taxonomy of Penicillium section Citrina

Similar Items