Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii

We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted...

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Main Authors:	Strenkert, Daniela, Schmollinger, Stefan, Schroda, Michael
Format:	Online
Language:	English
Published:	BioMed Central 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3225300/

id	pubmed-3225300
recordtype	oai_dc
spelling	pubmed-32253002011-11-29 Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii Strenkert, Daniela Schmollinger, Stefan Schroda, Michael Methodology We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR. BioMed Central 2011-11-03 /pmc/articles/PMC3225300/ /pubmed/22050920 http://dx.doi.org/10.1186/1746-4811-7-35 Text en Copyright ©2011 Strenkert et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Strenkert, Daniela Schmollinger, Stefan Schroda, Michael
spellingShingle	Strenkert, Daniela Schmollinger, Stefan Schroda, Michael Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
author_facet	Strenkert, Daniela Schmollinger, Stefan Schroda, Michael
author_sort	Strenkert, Daniela
title	Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
title_short	Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
title_full	Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
title_fullStr	Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
title_full_unstemmed	Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
title_sort	protocol: methodology for chromatin immunoprecipitation (chip) in chlamydomonas reinhardtii
description	We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.
publisher	BioMed Central
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3225300/
_version_	1611490342404620288

Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii

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