Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run

Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demo...

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Main Authors:	Riley, Matthew C., Lee, James Eric, Lesho, Emil, Kirkup, Benjamin C.
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221658/

id	pubmed-3221658
recordtype	oai_dc
spelling	pubmed-32216582011-11-30 Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run Riley, Matthew C. Lee, James Eric Lesho, Emil Kirkup, Benjamin C. Research Article Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production. Public Library of Science 2011-11-21 /pmc/articles/PMC3221658/ /pubmed/22132087 http://dx.doi.org/10.1371/journal.pone.0027085 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Riley, Matthew C. Lee, James Eric Lesho, Emil Kirkup, Benjamin C.
spellingShingle	Riley, Matthew C. Lee, James Eric Lesho, Emil Kirkup, Benjamin C. Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
author_facet	Riley, Matthew C. Lee, James Eric Lesho, Emil Kirkup, Benjamin C.
author_sort	Riley, Matthew C.
title	Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_short	Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_full	Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_fullStr	Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_full_unstemmed	Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_sort	optically mapping multiple bacterial genomes simultaneously in a single run
description	Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221658/
_version_	1611489383543734272

Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run

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