Roles for α2P24 and Copi in Endoplasmic Reticulum Cargo Exit Site Formation
A two-step reconstitution system for the generation of ER cargo exit sites from starting ER-derived low density microsomes (LDMs; 1.17 g/cc) is described. The first step is mediated by the hydrolysis of Mg2+ATP and Mg2+GTP, leading to the formation of a transitional ER (tER) with the soluble cargo a...
Main Authors: | , , , , , , |
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Format: | Online |
Language: | English |
Published: |
The Rockefeller University Press
1999
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206572/ |