High-throughput Saccharification Assay for Lignocellulosic Materials

Polysaccharides that make up plant lignocellulosic biomass can be broken down to produce a range of sugars that subsequently can be used in establishing a biorefinery. These raw materials would constitute a new industrial platform, which is both sustainable and carbon neutral, to replace the current...

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Main Authors:	Gomez, Leonardo D., Whitehead, Caragh, Roberts, Philip, McQueen-Mason, Simon J.
Format:	Online
Language:	English
Published:	MyJove Corporation 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196164/

id	pubmed-3196164
recordtype	oai_dc
spelling	pubmed-31961642011-10-24 High-throughput Saccharification Assay for Lignocellulosic Materials Gomez, Leonardo D. Whitehead, Caragh Roberts, Philip McQueen-Mason, Simon J. Molecular Biology Polysaccharides that make up plant lignocellulosic biomass can be broken down to produce a range of sugars that subsequently can be used in establishing a biorefinery. These raw materials would constitute a new industrial platform, which is both sustainable and carbon neutral, to replace the current dependency on fossil fuel. The recalcitrance to deconstruction observed in lignocellulosic materials is produced by several intrinsic properties of plant cell walls. Crystalline cellulose is embedded in matrix polysaccharides such as xylans and arabinoxylans, and the whole structure is encased by the phenolic polymer lignin, that is also difficult to digest 1. In order to improve the digestibility of plant materials we need to discover the main bottlenecks for the saccharification of cell walls and also screen mutant and breeding populations to evaluate the variability in saccharification 2. These tasks require a high throughput approach and here we present an analytical platform that can perform saccharification analysis in a 96-well plate format. This platform has been developed to allow the screening of lignocellulose digestibility of large populations from varied plant species. We have scaled down the reaction volumes for gentle pretreatment, partial enzymatic hydrolysis and sugar determination, to allow large numbers to be assessed rapidly in an automated system. MyJove Corporation 2011-07-03 /pmc/articles/PMC3196164/ /pubmed/21750494 http://dx.doi.org/10.3791/3240 Text en Copyright © 2011, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Gomez, Leonardo D. Whitehead, Caragh Roberts, Philip McQueen-Mason, Simon J.
spellingShingle	Gomez, Leonardo D. Whitehead, Caragh Roberts, Philip McQueen-Mason, Simon J. High-throughput Saccharification Assay for Lignocellulosic Materials
author_facet	Gomez, Leonardo D. Whitehead, Caragh Roberts, Philip McQueen-Mason, Simon J.
author_sort	Gomez, Leonardo D.
title	High-throughput Saccharification Assay for Lignocellulosic Materials
title_short	High-throughput Saccharification Assay for Lignocellulosic Materials
title_full	High-throughput Saccharification Assay for Lignocellulosic Materials
title_fullStr	High-throughput Saccharification Assay for Lignocellulosic Materials
title_full_unstemmed	High-throughput Saccharification Assay for Lignocellulosic Materials
title_sort	high-throughput saccharification assay for lignocellulosic materials
description	Polysaccharides that make up plant lignocellulosic biomass can be broken down to produce a range of sugars that subsequently can be used in establishing a biorefinery. These raw materials would constitute a new industrial platform, which is both sustainable and carbon neutral, to replace the current dependency on fossil fuel. The recalcitrance to deconstruction observed in lignocellulosic materials is produced by several intrinsic properties of plant cell walls. Crystalline cellulose is embedded in matrix polysaccharides such as xylans and arabinoxylans, and the whole structure is encased by the phenolic polymer lignin, that is also difficult to digest 1. In order to improve the digestibility of plant materials we need to discover the main bottlenecks for the saccharification of cell walls and also screen mutant and breeding populations to evaluate the variability in saccharification 2. These tasks require a high throughput approach and here we present an analytical platform that can perform saccharification analysis in a 96-well plate format. This platform has been developed to allow the screening of lignocellulose digestibility of large populations from varied plant species. We have scaled down the reaction volumes for gentle pretreatment, partial enzymatic hydrolysis and sugar determination, to allow large numbers to be assessed rapidly in an automated system.
publisher	MyJove Corporation
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196164/
_version_	1611481837881786368

High-throughput Saccharification Assay for Lignocellulosic Materials

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