Distinction of nucleobases – a tip-enhanced Raman approach

The development of novel DNA sequencing methods is one of the ongoing challenges in various fields of research seeking to address the demand for sequence information. However, many of these techniques rely on some kind of labeling or amplification steps. Here we investigate the intrinsic properties...

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Main Authors:	Treffer, Regina, Lin, Xiumei, Bailo, Elena, Deckert-Gaudig, Tanja, Deckert, Volker
Format:	Online
Language:	English
Published:	Beilstein-Institut 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190632/

id	pubmed-3190632
recordtype	oai_dc
spelling	pubmed-31906322011-10-14 Distinction of nucleobases – a tip-enhanced Raman approach Treffer, Regina Lin, Xiumei Bailo, Elena Deckert-Gaudig, Tanja Deckert, Volker Full Research Paper The development of novel DNA sequencing methods is one of the ongoing challenges in various fields of research seeking to address the demand for sequence information. However, many of these techniques rely on some kind of labeling or amplification steps. Here we investigate the intrinsic properties of tip-enhanced Raman scattering (TERS) towards the development of a novel, label-free, direct sequencing method. It is known that TERS allows the acquisition of spectral information with high lateral resolution and single-molecule sensitivity. In the presented experiments, single stranded adenine and uracil homopolymers were immobilized on different kinds of substrates (mica and gold nanoplates) and TERS experiments were conducted, which demonstrated the reproducibility of the technique. To elucidate the signal contributions from the specific nucleobases, TERS spectra were collected on single stranded calf thymus DNA with arbitrary sequence. The results show that, while the Raman signals with respect to the four nucleobases differ remarkably, specific markers can be determined for each respective base. The combination of sensitivity and reproducibility shows that the crucial demands for a sequencing procedure are met. Beilstein-Institut 2011-09-23 /pmc/articles/PMC3190632/ /pubmed/22003468 http://dx.doi.org/10.3762/bjnano.2.66 Text en Copyright © 2011, Treffer et al; licensee Beilstein-Institut. http://www.beilstein-journals.org/bjnano This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (http://www.beilstein-journals.org/bjnano)
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Treffer, Regina Lin, Xiumei Bailo, Elena Deckert-Gaudig, Tanja Deckert, Volker
spellingShingle	Treffer, Regina Lin, Xiumei Bailo, Elena Deckert-Gaudig, Tanja Deckert, Volker Distinction of nucleobases – a tip-enhanced Raman approach
author_facet	Treffer, Regina Lin, Xiumei Bailo, Elena Deckert-Gaudig, Tanja Deckert, Volker
author_sort	Treffer, Regina
title	Distinction of nucleobases – a tip-enhanced Raman approach
title_short	Distinction of nucleobases – a tip-enhanced Raman approach
title_full	Distinction of nucleobases – a tip-enhanced Raman approach
title_fullStr	Distinction of nucleobases – a tip-enhanced Raman approach
title_full_unstemmed	Distinction of nucleobases – a tip-enhanced Raman approach
title_sort	distinction of nucleobases – a tip-enhanced raman approach
description	The development of novel DNA sequencing methods is one of the ongoing challenges in various fields of research seeking to address the demand for sequence information. However, many of these techniques rely on some kind of labeling or amplification steps. Here we investigate the intrinsic properties of tip-enhanced Raman scattering (TERS) towards the development of a novel, label-free, direct sequencing method. It is known that TERS allows the acquisition of spectral information with high lateral resolution and single-molecule sensitivity. In the presented experiments, single stranded adenine and uracil homopolymers were immobilized on different kinds of substrates (mica and gold nanoplates) and TERS experiments were conducted, which demonstrated the reproducibility of the technique. To elucidate the signal contributions from the specific nucleobases, TERS spectra were collected on single stranded calf thymus DNA with arbitrary sequence. The results show that, while the Raman signals with respect to the four nucleobases differ remarkably, specific markers can be determined for each respective base. The combination of sensitivity and reproducibility shows that the crucial demands for a sequencing procedure are met.
publisher	Beilstein-Institut
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190632/
_version_	1611480035296804864

Distinction of nucleobases – a tip-enhanced Raman approach

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