Effects of incomplete decay in fluorescence lifetime estimation

Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can supe...

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Bibliographic Details
Main Authors: Leung, Regina Won Kay, Yeh, Shu-Chi Allison, Fang, Qiyin
Format: Online
Language:English
Published: Optical Society of America 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184861/