Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat
To assess the ability of traditional Chinese medicine Cistanches Herba extract (CHE) to prevent bone loss in the ovariectomized (OVX) rat, Cistanches Herba extract (CHE) was administered intragastrically to the rats. Female rats were anesthetized with pentobarbital sodium (40 mg kg−1, i.p.), and the...
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Molecular Diversity Preservation International (MDPI)
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179152/ |
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pubmed-31791522011-09-27 Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat Liang, Haidong Yu, Fang Tong, Zhihong Huang, Zaiguo Article To assess the ability of traditional Chinese medicine Cistanches Herba extract (CHE) to prevent bone loss in the ovariectomized (OVX) rat, Cistanches Herba extract (CHE) was administered intragastrically to the rats. Female rats were anesthetized with pentobarbital sodium (40 mg kg−1, i.p.), and their ovaries were removed bilaterally. The rats in the sham-operated group were anesthetized, laparotomized, and sutured without removing their ovaries. After 1 week of recovery from surgery, the OVX rats were randomly divided into three groups and orally treated with H2O (OVX group) or CHE (100 or 200 mg kg−1 daily) for 3 months. The sham-operated group (n = 8) was orally treated with H2O. After 3 months, the total body bone mineral density (BMD), bone mineral content (BMC), Bone biomechanical index, blood mineral levels and blood antioxidant enzymes activities were examined in sham-operated, ovariectomized and Cistanches Herba extract treated rats. Results showed that Cistanches Herba extract treatment significantly dose-dependently enhanced bone mineral density (BMD), bone mineral content (BMC), maximum load, displacement at maximum load, stress at maximum load, load at auto break, displacement at auto break, and stress at auto break, and blood antioxidant enzymes activities, decreased blood Ca, Zn and Cu levels compared to the OVX group. This experiment demonstrates that the administration of Cistanches Herba extract to ovariectomized rats reverses bone loss and prevents osteoporosis. Molecular Diversity Preservation International (MDPI) 2011-08-08 /pmc/articles/PMC3179152/ /pubmed/21954345 http://dx.doi.org/10.3390/ijms12085060 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Liang, Haidong Yu, Fang Tong, Zhihong Huang, Zaiguo |
| spellingShingle |
Liang, Haidong Yu, Fang Tong, Zhihong Huang, Zaiguo Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat |
| author_facet |
Liang, Haidong Yu, Fang Tong, Zhihong Huang, Zaiguo |
| author_sort |
Liang, Haidong |
| title |
Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat |
| title_short |
Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat |
| title_full |
Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat |
| title_fullStr |
Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat |
| title_full_unstemmed |
Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat |
| title_sort |
effect of cistanches herba aqueous extract on bone loss in ovariectomized rat |
| description |
To assess the ability of traditional Chinese medicine Cistanches Herba extract (CHE) to prevent bone loss in the ovariectomized (OVX) rat, Cistanches Herba extract (CHE) was administered intragastrically to the rats. Female rats were anesthetized with pentobarbital sodium (40 mg kg−1, i.p.), and their ovaries were removed bilaterally. The rats in the sham-operated group were anesthetized, laparotomized, and sutured without removing their ovaries. After 1 week of recovery from surgery, the OVX rats were randomly divided into three groups and orally treated with H2O (OVX group) or CHE (100 or 200 mg kg−1 daily) for 3 months. The sham-operated group (n = 8) was orally treated with H2O. After 3 months, the total body bone mineral density (BMD), bone mineral content (BMC), Bone biomechanical index, blood mineral levels and blood antioxidant enzymes activities were examined in sham-operated, ovariectomized and Cistanches Herba extract treated rats. Results showed that Cistanches Herba extract treatment significantly dose-dependently enhanced bone mineral density (BMD), bone mineral content (BMC), maximum load, displacement at maximum load, stress at maximum load, load at auto break, displacement at auto break, and stress at auto break, and blood antioxidant enzymes activities, decreased blood Ca, Zn and Cu levels compared to the OVX group. This experiment demonstrates that the administration of Cistanches Herba extract to ovariectomized rats reverses bone loss and prevents osteoporosis. |
| publisher |
Molecular Diversity Preservation International (MDPI) |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179152/ |
| _version_ |
1611477086642372608 |