A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner

Transcriptional gene silencing (TGS) can be induced by promoter-targeted small interfering RNA (siRNA). Long-distance transmission of TGS by viral infection in plants has been reported. However, systemic TGS has not been observed in the case of using an inverted repeat transgene as the silencing tri...

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Main Authors:	Bai, Songling, Kasai, Atsushi, Yamada, Kaori, Li, Tianzhong, Harada, Takeo
Format:	Online
Language:	English
Published:	Oxford University Press 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170550/

id	pubmed-3170550
recordtype	oai_dc
spelling	pubmed-31705502011-09-12 A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner Bai, Songling Kasai, Atsushi Yamada, Kaori Li, Tianzhong Harada, Takeo Research Papers Transcriptional gene silencing (TGS) can be induced by promoter-targeted small interfering RNA (siRNA). Long-distance transmission of TGS by viral infection in plants has been reported. However, systemic TGS has not been observed in the case of using an inverted repeat transgene as the silencing trigger. Here it is reported that a mobile signal, presumably the siRNA, produced from a hairpin structure transgene controlled by a companion cell-specific promoter can also induce transmissible TGS in both a modified agroinfiltration and a grafting system. Although the transmissible TGS occurred only in cells located in the vicinity of a leaf vein in the scion, very strong silencing was observed in the root system, especially the lateral roots, including the root apical meristem. The transmissible TGS was maintained through tissue culture and subsequently inherited by the progeny. The results suggest the potential application of mobile promoter-targeting siRNA in horticulture for improvement of plant cultivars by grafting. Oxford University Press 2011-08 2011-06-07 /pmc/articles/PMC3170550/ /pubmed/21652532 http://dx.doi.org/10.1093/jxb/err163 Text en © 2011 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bai, Songling Kasai, Atsushi Yamada, Kaori Li, Tianzhong Harada, Takeo
spellingShingle	Bai, Songling Kasai, Atsushi Yamada, Kaori Li, Tianzhong Harada, Takeo A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner
author_facet	Bai, Songling Kasai, Atsushi Yamada, Kaori Li, Tianzhong Harada, Takeo
author_sort	Bai, Songling
title	A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner
title_short	A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner
title_full	A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner
title_fullStr	A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner
title_full_unstemmed	A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner
title_sort	mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner
description	Transcriptional gene silencing (TGS) can be induced by promoter-targeted small interfering RNA (siRNA). Long-distance transmission of TGS by viral infection in plants has been reported. However, systemic TGS has not been observed in the case of using an inverted repeat transgene as the silencing trigger. Here it is reported that a mobile signal, presumably the siRNA, produced from a hairpin structure transgene controlled by a companion cell-specific promoter can also induce transmissible TGS in both a modified agroinfiltration and a grafting system. Although the transmissible TGS occurred only in cells located in the vicinity of a leaf vein in the scion, very strong silencing was observed in the root system, especially the lateral roots, including the root apical meristem. The transmissible TGS was maintained through tissue culture and subsequently inherited by the progeny. The results suggest the potential application of mobile promoter-targeting siRNA in horticulture for improvement of plant cultivars by grafting.
publisher	Oxford University Press
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170550/
_version_	1611474858631233536

A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner

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