Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression

Most studies on Salmonella enterica serovar Typhimurium infection focus on strains ATCC SL1344 or NTCC 12023 (ATCC 14028). We have compared the abilities of these strains to induce membrane ruffles and invade epithelial cells. S. Typhimurium strain 12023 is less invasive and induces smaller membrane...

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Main Authors:	Clark, Leann, Perrett, Charlotte A., Malt, Layla, Harward, Caryn, Humphrey, Suzanne, Jepson, Katy A., Martinez-Argudo, Isabel, Carney, Laura J., La Ragione, Roberto M., Humphrey, Tom J., Jepson, Mark A.
Format:	Online
Language:	English
Published:	Society for General Microbiology 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167892/

id	pubmed-3167892
recordtype	oai_dc
spelling	pubmed-31678922011-10-03 Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression Clark, Leann Perrett, Charlotte A. Malt, Layla Harward, Caryn Humphrey, Suzanne Jepson, Katy A. Martinez-Argudo, Isabel Carney, Laura J. La Ragione, Roberto M. Humphrey, Tom J. Jepson, Mark A. Microbial Pathogenicity Most studies on Salmonella enterica serovar Typhimurium infection focus on strains ATCC SL1344 or NTCC 12023 (ATCC 14028). We have compared the abilities of these strains to induce membrane ruffles and invade epithelial cells. S. Typhimurium strain 12023 is less invasive and induces smaller membrane ruffles on MDCK cells compared with SL1344. Since the SPI-1 effector SopE is present in SL1344 and absent from 12023, and SL1344 sopE mutants have reduced invasiveness, we investigated whether 12023 is less invasive due to the absence of SopE. However, comparison of SopE+ and SopE− S. Typhimurium strains, sopE deletion mutants and 12023 expressing a sopE plasmid revealed no consistent relationship between SopE status and relative invasiveness. Nevertheless, absence of SopE was closely correlated with reduced size of membrane ruffles. A PprgH–gfp reporter revealed that relatively few of the 12023 population (and that of the equivalent strain ATCC 14028) express SPI-1 compared to other S. Typhimurium strains. Expression of a PhilA–gfp reporter mirrored that of PprgH–gfp in 12023 and SL1344, implicating reduced signalling via the transcription factor HilA in the heterogeneous SPI-1 expression of these strains. The previously unrecognized strain heterogeneity in SPI-1 expression and invasiveness has important implications for studies of Salmonella infection. Society for General Microbiology 2011-07 /pmc/articles/PMC3167892/ /pubmed/21493681 http://dx.doi.org/10.1099/mic.0.048496-0 Text en © 2011 SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Clark, Leann Perrett, Charlotte A. Malt, Layla Harward, Caryn Humphrey, Suzanne Jepson, Katy A. Martinez-Argudo, Isabel Carney, Laura J. La Ragione, Roberto M. Humphrey, Tom J. Jepson, Mark A.
spellingShingle	Clark, Leann Perrett, Charlotte A. Malt, Layla Harward, Caryn Humphrey, Suzanne Jepson, Katy A. Martinez-Argudo, Isabel Carney, Laura J. La Ragione, Roberto M. Humphrey, Tom J. Jepson, Mark A. Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression
author_facet	Clark, Leann Perrett, Charlotte A. Malt, Layla Harward, Caryn Humphrey, Suzanne Jepson, Katy A. Martinez-Argudo, Isabel Carney, Laura J. La Ragione, Roberto M. Humphrey, Tom J. Jepson, Mark A.
author_sort	Clark, Leann
title	Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression
title_short	Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression
title_full	Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression
title_fullStr	Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression
title_full_unstemmed	Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression
title_sort	differences in salmonella enterica serovar typhimurium strain invasiveness are associated with heterogeneity in spi-1 gene expression
description	Most studies on Salmonella enterica serovar Typhimurium infection focus on strains ATCC SL1344 or NTCC 12023 (ATCC 14028). We have compared the abilities of these strains to induce membrane ruffles and invade epithelial cells. S. Typhimurium strain 12023 is less invasive and induces smaller membrane ruffles on MDCK cells compared with SL1344. Since the SPI-1 effector SopE is present in SL1344 and absent from 12023, and SL1344 sopE mutants have reduced invasiveness, we investigated whether 12023 is less invasive due to the absence of SopE. However, comparison of SopE+ and SopE− S. Typhimurium strains, sopE deletion mutants and 12023 expressing a sopE plasmid revealed no consistent relationship between SopE status and relative invasiveness. Nevertheless, absence of SopE was closely correlated with reduced size of membrane ruffles. A PprgH–gfp reporter revealed that relatively few of the 12023 population (and that of the equivalent strain ATCC 14028) express SPI-1 compared to other S. Typhimurium strains. Expression of a PhilA–gfp reporter mirrored that of PprgH–gfp in 12023 and SL1344, implicating reduced signalling via the transcription factor HilA in the heterogeneous SPI-1 expression of these strains. The previously unrecognized strain heterogeneity in SPI-1 expression and invasiveness has important implications for studies of Salmonella infection.
publisher	Society for General Microbiology
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167892/
_version_	1611473967052226560

Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression

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