Koilocytes are enriched for alkaline-labile sites
This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA p...
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| Format: | Online |
| Language: | English |
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PAGEPress Publications
2010
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167319/ |
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pubmed-31673192011-11-09 Koilocytes are enriched for alkaline-labile sites Cortés-Gutiérrez, E.I. Dávila-Rodríguez, M.I. Fernández, J.L. López-Fernández, C. Gosálvez, J. Original Paper This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection. PAGEPress Publications 2010-12-21 /pmc/articles/PMC3167319/ /pubmed/21337807 http://dx.doi.org/10.4081/ejh.2010.e32 Text en ©Copyright E.I. Cortés-Gutiérrez et al., 2010 This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0). Licensee PAGEPress, Italy |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Cortés-Gutiérrez, E.I. Dávila-Rodríguez, M.I. Fernández, J.L. López-Fernández, C. Gosálvez, J. |
| spellingShingle |
Cortés-Gutiérrez, E.I. Dávila-Rodríguez, M.I. Fernández, J.L. López-Fernández, C. Gosálvez, J. Koilocytes are enriched for alkaline-labile sites |
| author_facet |
Cortés-Gutiérrez, E.I. Dávila-Rodríguez, M.I. Fernández, J.L. López-Fernández, C. Gosálvez, J. |
| author_sort |
Cortés-Gutiérrez, E.I. |
| title |
Koilocytes are enriched for alkaline-labile sites |
| title_short |
Koilocytes are enriched for alkaline-labile sites |
| title_full |
Koilocytes are enriched for alkaline-labile sites |
| title_fullStr |
Koilocytes are enriched for alkaline-labile sites |
| title_full_unstemmed |
Koilocytes are enriched for alkaline-labile sites |
| title_sort |
koilocytes are enriched for alkaline-labile sites |
| description |
This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection. |
| publisher |
PAGEPress Publications |
| publishDate |
2010 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167319/ |
| _version_ |
1611473800738635776 |