A rapid and easy method for the DNA extraction from Cryptococcus neoformans

A rapid and easy method for the DNA extraction from Cryptococcus neoformans

DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported meth...

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Main Authors: Mseddi, Fatma, Jarboui, Mohammed Ali, Sellami, Amira, Sellami, Hayet, Ayadi, Ali
Format: Online
Language:English
Published: BioMed Central 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156736/
id pubmed-3156736
recordtype oai_dc
spelling pubmed-31567362011-08-17 A rapid and easy method for the DNA extraction from Cryptococcus neoformans Mseddi, Fatma Jarboui, Mohammed Ali Sellami, Amira Sellami, Hayet Ayadi, Ali Research DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods. BioMed Central 2011-07-21 /pmc/articles/PMC3156736/ /pubmed/21777412 http://dx.doi.org/10.1186/1480-9222-13-5 Text en Copyright ©2011 Mseddi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Mseddi, Fatma
Jarboui, Mohammed Ali
Sellami, Amira
Sellami, Hayet
Ayadi, Ali
spellingShingle Mseddi, Fatma
Jarboui, Mohammed Ali
Sellami, Amira
Sellami, Hayet
Ayadi, Ali
A rapid and easy method for the DNA extraction from Cryptococcus neoformans
author_facet Mseddi, Fatma
Jarboui, Mohammed Ali
Sellami, Amira
Sellami, Hayet
Ayadi, Ali
author_sort Mseddi, Fatma
title A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_short A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_full A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_fullStr A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_full_unstemmed A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_sort rapid and easy method for the dna extraction from cryptococcus neoformans
description DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods.
publisher BioMed Central
publishDate 2011
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156736/
_version_ 1611471035657355264

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