A One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles

Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format. In addition, alkali minipreps usually require additional column-based purification steps and cannot isolate other extra-chromosoma...

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Bibliographic Details
Main Authors: Lezin, George, Kosaka, Yasuhiro, Yost, H. Joseph, Kuehn, Michael R., Brunelli, Luca
Format: Online
Language:English
Published: Public Library of Science 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156146/